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  4. Recombinant Drosophila melanogaster Serine/threonine-protein kinase Doa (Doa), partial

Recombinant Drosophila melanogaster Serine/threonine-protein kinase Doa (Doa), partial

  • 中文名稱：
    黑腹果蠅Doa重組蛋白
  • 貨號(hào)：
    CSB-YP344218DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Doa重組蛋白
  • 貨號(hào)：
    CSB-EP344218DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Doa重組蛋白
  • 貨號(hào)：
    CSB-EP344218DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Doa重組蛋白
  • 貨號(hào)：
    CSB-BP344218DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Doa重組蛋白
  • 貨號(hào)：
    CSB-MP344218DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Doa
  • Uniprot No.：
  • 別名：
    Doa; CG42320Serine/threonine-protein kinase Doa; EC 2.7.12.1; Protein darkener of apricot
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Negative regulator of the copia retrotransposon element of the white (w) gene. In the eye, it is required for normal pigmentation, photoreceptor cell development and for organization of interommatidial bristles. Also essential for embryonic segmentation and differentiation of the nervous system. Functions in the control of alternative splicing by phosphorylating the arginine/serine-rich splicing factors, SR proteins.
  • 基因功能參考文獻(xiàn)：
    1. Doa could modulate pheromone production and sex behavior by altering sexual differentiation in the cuticle and the nervous system PMID: 28088352
    2. Strong female-to-male sex transformations in double mutants of the IMD pathway in combination with Doa alleles, is reported. PMID: 26434917
    3. DOA protein and its substrate EF-1gamma regulate organelle transport along microtubules. PMID: 24163433
    4. LAMMER/Cdc2-like kinase, represented by Doa and its mammalian homolog CLK2, is a critical and conserved component in the regulatory program of the mitosis-to-meiosis switch PMID: 23376537
    5. The Doa locus encodes six different kinases, of which a 69-kDa isoform is expressed solely in females. PMID: 20876993
    6. DNA mutational analysis was done on Doa - a gene required for oocyte specification and development. PMID: 12930750
    7. Data indicate that genes encoding proteins involved in closely related pathways are concentrated at certain loci to achieve more efficient regulation of their expression as exemplified from distribution of DOA protein on Drosophila polythene chromosomes. PMID: 16964964
    8. DX16 could be a novel serine-arginine-rich protein phosphorylated by DOA and it may participate in the formation of splicing complex through its interactions with other splicing related proteins. PMID: 17828581
    9. Alternative promoter usage generates multiple evolutionarily conserved isoforms of Drosophila DOA kinase. PMID: 18327787
    10. the roles of the multiple isoforms, we mapped polymorphisms, deletions, and P-element insertions in the locus, identifying several that are largely, if not completely, isoform specific in their effects PMID: 18660538
    11. mutations in the gene protect against the decrease in life span caused by the reactive oxygen species generator paraquat, associated with an increase in cytoplasmic and mitochondrial superoxide dismutase protein levels and activity PMID: 19135146

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  • 亞細(xì)胞定位：
    [Isoform C]: Cytoplasm, cytosol.; [Isoform A]: Nucleus. Note=Mainly nuclear with only low levels present in the cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily
  • 組織特異性：
    Ubiquitous expression in embryos. Stage 17 embryos show elevated expression in CNS and brain. Ubiquitous expression in larval imaginal disks. Increased expression posterior to the eye-antennal disk morphogenetic furrow.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG42320

    STRING: 7227.FBpp0289029

    UniGene: Dm.6767



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