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Recombinant Drosophila melanogaster Protein maelstrom (mael)

  • 中文名稱:
    黑腹果蠅mael重組蛋白
  • 貨號:
    CSB-YP893783DLU
  • 說明書:
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅mael重組蛋白
  • 貨號:
    CSB-EP893783DLU
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅mael重組蛋白
  • 貨號:
    CSB-EP893783DLU-B
  • 說明書:
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅mael重組蛋白
  • 貨號:
    CSB-BP893783DLU
  • 說明書:
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅mael重組蛋白
  • 貨號:
    CSB-MP893783DLU
  • 說明書:
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    mael
  • Uniprot No.:
  • 別名:
    mael; CG11254Protein maelstrom
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-459
  • 氨基酸序列
    MAPKKHSGFM MFVNEWRNRN AEGRRMTLAQ AVSHCGTIWE KMNTQQRGPY NSGGKDANVA QRAKRESSNG HGQVDKAQRE ATESLMDMKR TIERLVLNAK MSHDLENAKF VFVAFNYFTK ALTTDVYVPA EFAACEYSLK EGIRSIYSTM IDPGQIIFGQ GSDALLHSST THDLPLPPNA LGEKNMTKLY RNIVDYLSKC QGKGKTLVVF TPAENITMVK SCFRYLECDD DFRDGGEKIQ VFDIQYLLFI LKKEVMNVAD LNDEKINKFA TDAFFKKDFF EFTAGIACQY HEDNDRTKYC TQSMVTRWAY TFTDFMCGDL AITVQPGKHI PAQTKPNYLI ISSYASSLDH ESSFDSFYSL PGSGVKKESQ PEACSLSSSR LSVASSSYKP IDHTSFAANL NEVSEFPSLG MRNSSKHHGI AASAQREWNA RNLPTHSRLI RKVSDNDFSV NGADGKLKK
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Involved both in the piRNA and miRNA metabolic processes. As a component of the meiotic nuage, plays a central role during oogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Repression of transposable elements is mediated via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the repression of transposons. As a nuclear component, it is required for proper differentiation in the germline stem cell (GSC) lineage by repressing microRNA-7 (miR-7), thereby acting as an indirect regulator of bag-of-marbles (Bam). Acts by binding to the promoter of miR-7 gene and repressing its expression; miR-7 repression alleviates the Bam repression by miR-7, thereby allowing differentiation in the germline stem cell (GSC) lineage. Indirectly required to position the microtubule organizing center in stage 2-6 oocytes.
  • 基因功能參考文獻:
    1. Study shows that the HMG protein Maelstrom is essential for Piwi-mediated silencing in Drosophila. PMID: 23159368
    2. Mael serves as a platform that nucleates other microtubule-organizing center components to form a functional MTOC in early oocyte development PMID: 22085963
    3. characterized a null allele of maelstrom (mael) and showed that PMID: 12538514
    4. Mael ensures proper differentiation in the germline stem cell lineage by repressing miR-7 PMID: 19758565
  • 亞細胞定位:
    Cytoplasm. Nucleus. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Vas, aub and spn-E are required for nuage localization. Shuttles between the cytoplasm and the nucleus.
  • 蛋白家族:
    Maelstrom family
  • 組織特異性:
    In germaria and egg chambers, it is detected in the germline. In the germarium, it is in all regions, including region I where the germ cells are dividing. In early egg chambers, it is uniformly distributed throughout the nurse cells and oocyte but, by st
  • 數據庫鏈接:

    KEGG: dme:Dmel_CG11254

    STRING: 7227.FBpp0088574

    UniGene: Dm.5827



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