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  4. Recombinant Drosophila melanogaster Protein four-jointed (fj), partial

Recombinant Drosophila melanogaster Protein four-jointed (fj), partial

  • 中文名稱：
    黑腹果蠅fj重組蛋白
  • 貨號：
    CSB-YP347491DLU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅fj重組蛋白
  • 貨號：
    CSB-EP347491DLU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅fj重組蛋白
  • 貨號：
    CSB-EP347491DLU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅fj重組蛋白
  • 貨號：
    CSB-BP347491DLU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅fj重組蛋白
  • 貨號：
    CSB-MP347491DLU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    fj
  • Uniprot No.：
  • 別名：
    fj; CG10917Extracellular serine/threonine protein kinase four-jointed; EC 2.7.11.1) [Cleaved into: Protein four-jointed; secreted isoform]
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Golgi serine/threonine protein kinase required for intermediate growth in the proximal-distal axis. Phosphorylates specific residues within extracellular cadherin domains of Fat (ft) and Dachsous (ds) as they transit through the Golgi. Acts in ommatidial polarity determination as a secondary signal downstream of Notch, JAK/STAT and wingless. Also necessary for the initiation, up-regulation or maintenance of Notch ligand, Serrate (Ser) expression in legs, thereby participating in a feedback loop with N signaling. Sufficient for joint formation and growth in the leg.
  • 基因功能參考文獻：
    1. Four-jointed acts both on Fat to promote its binding to Dachsous and on Dachsous to inhibit its binding to Fat, with a bias towards a stronger effect on Fat PMID: 25707557
    2. modulates fat:daschsous binding and polarization activity via phosphorylation site PMID: 20434335
    3. effects on wing size and planar polarity by phosphorylating fat (ft) and dachsous (ds) activity PMID: 20434337
    4. The gradient of Fj and Ds expression provide partially redundant positional information essential for specifying the polarization. axis PMID: 15548581
    5. Wnt oncogene analog 4 antagonizes the polarizing effect of Fj PMID: 15986451

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  • 亞細胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein. Note=Golgi apparatus, in the wing pouch.; [Protein four-jointed, secreted isoform]: Secreted.
  • 蛋白家族：
    FJX1/FJ family
  • 組織特異性：
    In the eye disk, expressed in a gradient ahead of the morphogenetic furrow, high at the equator and low at the poles of the eye. In the leg disk, expressed in concentric rings, possibly corresponding to segmental boundaries. In the wing disk, expression i
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG10917

    STRING: 7227.FBpp0085933

    UniGene: Dm.4763



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