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  4. Recombinant Drosophila melanogaster Protein cutoff (cuff)

Recombinant Drosophila melanogaster Protein cutoff (cuff)

  • 中文名稱：
    Recombinant Drosophila melanogaster Protein cutoff(cuff)
  • 貨號：
    CSB-YP896050DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Protein cutoff(cuff)
  • 貨號：
    CSB-EP896050DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Protein cutoff(cuff)
  • 貨號：
    CSB-EP896050DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Protein cutoff(cuff)
  • 貨號：
    CSB-BP896050DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Protein cutoff(cuff)
  • 貨號：
    CSB-MP896050DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cuff
  • Uniprot No.：
  • 別名：
    CG13190; CG13190 gene product from transcript CG13190-RA; CG13190 PA; cuff; Cuff PA; CUFF_DROME; Cutoff; Dmel\CG13190; Protein cutoff
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-384
  • 氨基酸序列
    MNSNYTILNI RAHSWAEKDN LVFPTITKPV SNGWFAWSPI GQFEPNSQKV PYVVVPPSIK FPISLRYKDS PPKLEKKPNI FLDNMLQYIE SSSYMYVMVR NNQQAQLNAN IVCTSEVLEL MMCAPYEKKT GWSLGVTRYR NTMYICRIDV EQPDPIDQDN LKRAMQEFWL RNLRTHCVFE NGIKMHQHNQ SSEEHLRFHG VFSFDLNGNR VLFDSPVLAE MPSTTLNGSA LSWVDLQIRP MFMSRLDWPE HNRTEALKWW VKCFLLGIES LYIARRDENA HVHNIEKTLV RDLWKSCEKD WSPTVCANFM IYLLNCISQV MAPIDCPSTV YLFQFDASQG TVSYKGLRGR NQYTFVSDWF RMMLDDHTND MCKTPNLQTM SSIV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the piRNA pathway in germline. tissues.
  • 基因功能參考文獻：
    1. Results confirm that Cutoff is involved in the transcriptional regulation of piRNA clusters and in the repression of transposable elements in germ cells. Surprisingly, however, Cutoff is found enriched at and affects the expression of other noncoding RNAs, including spliceosomal RNAs and small nucleolar RNAs. Finally, mutations in Cutoff result in the deregulation of hundreds of protein-coding genes in germ cells. PMID: 28420675
    2. Cutoff suppresses RNA polymerase II termination to ensure expression of piRNA precursors. PMID: 27292797
    3. results unveil a link between Cuff activity, heterochromatin assembly and piRNA cluster expression, which is critical for stem-cell and germ-cell development in Drosophila PMID: 21952049
  • 亞細胞定位：
    Cytoplasm. Chromosome. Note=Localised to perinuclear puncta in the nurse cells of younger egg chambers. Accumulates at centromeric/pericentromeric positions in germ-cell nuclei and strongly colocalizes with the major heterochromatic domains.
  • 蛋白家族：
    DXO/Dom3Z family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG13190

    STRING: 7227.FBpp0087141

    UniGene: Dm.30905



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