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  4. Recombinant Drosophila melanogaster Protein claret segregational (ncd), partial

Recombinant Drosophila melanogaster Protein claret segregational (ncd), partial

  • 中文名稱：
    黑腹果蠅ncd重組蛋白
  • 貨號(hào)：
    CSB-YP324046DLU
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅ncd重組蛋白
  • 貨號(hào)：
    CSB-EP324046DLU
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅ncd重組蛋白
  • 貨號(hào)：
    CSB-EP324046DLU-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅ncd重組蛋白
  • 貨號(hào)：
    CSB-BP324046DLU
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅ncd重組蛋白
  • 貨號(hào)：
    CSB-MP324046DLU
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ncd
  • Uniprot No.：
  • 別名：
    ncd; CA(ND); CG7831; Protein claret segregational
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    NCD is required for normal chromosomal segregation in meiosis, in females, and in early mitotic divisions of the embryo. The NCD motor activity is directed toward the microtubule's minus end.
  • 基因功能參考文獻(xiàn)：
    1. In vitro experiments using the drosophila kinesin-14, non-claret disjunctional (Ncd) show that the diffusive anchorage indeed impacts the force that kinesin-14 motors can generate between two microtubules and that modifications of the anchorage interfaces may act as a regulatory factor influencing the force generation by kinesin-14. PMID: 29880831
    2. 14-3-3 translates a spatial cue provided by Aurora B to target Ncd selectively to the spindle within the large volume of oocytes. PMID: 28860275
    3. Drosophila Ncd reveals an evolutionarily conserved powerstroke mechanism for homodimeric and heterodimeric kinesin-14s. PMID: 25941402
    4. Mutation of an invariant residue in loop L7 of the central beta-sheet of the Drosophila kinesin-14 Ncd motor alters both nucleotide and microtubule binding, although the mutated residue is not present in either site. PMID: 23077560
    5. Data suggest that the C-terminal residues of Ncd play an important role in modulating the interaction of the motor with the microtubule. PMID: 22449971
    6. Ensemble averaging for each event category revealed that plus- and minus-directed strokes have different size and occur at different instants within the ncd-microtubules attachment sequence. PMID: 21156132
    7. mutation of a single residue in the kinesin-14 Ncd causes the motor to release ADP and hydrolyze ATP faster than wild type PMID: 20602775
    8. Stable neck-core interactions in ncd generate a binding geometry between motor & microtubule, placing motor ahead of cargo in the minus-end direction. After ATP uptake, the heads rearrange, promoting a swing of the neck in the minus-end direction. PMID: 12426369
    9. results show that the nucleotide-binding site of kinesin-family motors closes when the motor.diphosphate complex binds to microtubules PMID: 12730601
    10. Spindle assembly occurs in an ncd mutant, but interactions between microtubule-coated chromosomes are unstable; thus Ncd movement along microtubules is needed to stabilize interactions between chromosomes. PMID: 15797926
    11. a bidirectional Drosophila Kinesin-14 motor that moves either to the microtubule plus or minus end in vitro unexpectedly causes only minor spindle defects in vivo PMID: 16190984
    12. results show that the force-producing conformational change in Ncd occurs on ATP binding, as in other kinesins, but involves the swing of a lever-arm mechanical element similar to that described for myosins PMID: 16382238
    13. These data demonstrate generation of sliding forces between adjacent microtubules by Ncd, and they confirm the proposed roles of kinesins-14 in the mitotic spindle morphogenesis. PMID: 17596520
    14. Motility of a truncation series of Ncd and removal of the tubulin tail suggested that the Ncd tail serves as an electrostatic tether to microtubules. PMID: 18207739
    15. The tail of ncd is essential for binding to spindles and centrosomes, but both the head and tail are needed for normal spindle assembly and function. PMID: 18957509
    16. Results show that kinesin-14 causes sliding and expansion of anti-parallel microtubules by interactions through the motor domain on one side and the tail domain on the other, and that this accounts for the roles of kinesin-14 in spindle organization. PMID: 19430467

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  • 亞細(xì)胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, NCD subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG7831

    STRING: 7227.FBpp0084900

    UniGene: Dm.6668



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