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Recombinant Drosophila melanogaster NEDD8 (Nedd8)

  • 中文名稱:
    黑腹果蠅Nedd8重組蛋白
  • 貨號:
    CSB-YP893288DLU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅Nedd8重組蛋白
  • 貨號:
    CSB-EP893288DLU
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅Nedd8重組蛋白
  • 貨號:
    CSB-EP893288DLU-B
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅Nedd8重組蛋白
  • 貨號:
    CSB-BP893288DLU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅Nedd8重組蛋白
  • 貨號:
    CSB-MP893288DLU
  • 說明書:
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    Nedd8
  • Uniprot No.:
  • 別名:
    Nedd8; CG10679; NEDD8; Neddylin; Ubiquitin-like protein Nedd8
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    full length protein
  • 表達(dá)區(qū)域:
    1-76
  • 氨基酸序列
    MLIKVKTLTG KEIEIDIEPT DKVDRIKERV EEKEGIPPQQ QRLIFSGKQM NDDKTAADYK VQGGSVLHLV LALRGG
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    Ubiquitin-like protein which plays an important role in cell cycle control, embryogenesis and neurogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex Uba3-Ula1 and linkage to the E2 enzyme UbcE2M. Attachment of Nedd8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  • 基因功能參考文獻(xiàn):
    1. the Cul1-based SCF complex requires Nedd8 modification for the degradation processes of Ci, Arm, and CycE in vivo PMID: 12231629
    2. Genetic analyses indicate that a balance between neddylation and deneddylation maintained by DEN1 is crucial for animal viability. PMID: 18782863
  • 亞細(xì)胞定位:
    Nucleus.
  • 蛋白家族:
    Ubiquitin family
  • 數(shù)據(jù)庫鏈接:

    KEGG: dme:Dmel_CG10679

    STRING: 7227.FBpp0080733

    UniGene: Dm.30848



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