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  4. Recombinant Drosophila melanogaster Homeobox protein homothorax (hth)

Recombinant Drosophila melanogaster Homeobox protein homothorax (hth)

  • 中文名稱：
    黑腹果蠅hth重組蛋白
  • 貨號：
    CSB-YP523699DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅hth重組蛋白
  • 貨號：
    CSB-EP523699DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅hth重組蛋白
  • 貨號：
    CSB-EP523699DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅hth重組蛋白
  • 貨號：
    CSB-BP523699DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅hth重組蛋白
  • 貨號：
    CSB-MP523699DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hth
  • Uniprot No.：
  • 別名：
    hth; dtl; CG17117; Homeobox protein homothorax; Homeobox protein dorsotonals
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-487
  • 氨基酸序列
    MAQPRYDDGL HGYGMDSGAA AAAMYDPHAG HRPPGLQGLP SHHSPHMTHA AAAAATVGMH GYHSGAGGHG TPSHVSPVGN HLMGAIPEVH KRDKDAIYEH PLFPLLALIF EKCELATCTP REPGVQGGDV CSSESFNEDI AMFSKQIRSQ KPYYTADPEV DSLMVQAIQV LRFHLLELEK VHELCDNFCH RYISCLKGKM PIDLVIDERD TTKPPELGSA NGEGRSNADS TSHTDGASTP DVRPPSSSLS YGGAMNDDAR SPGAGSTPGP LSQQPPALDT SDPDGKFLSS LNPSELTYDG RWCRREWSSP ADARNADASR RLYSSVFLGS PDNFGTSASG DASNASIGSG EGTGEEDDDA SGKKNQKKRG IFPKVATNIL RAWLFQHLTH PYPSEDQKKQ LAQDTGLTIL QVNNWFINAR RRIVQPMIDQ SNRAVYTPHP GPSGYGHDAM GYMMDSQAHM MHRPPGDPGF HQGYPHYPPA EYYGQHL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    All isoforms are required for patterning of the embryonic cuticle. Acts with exd to delimit the eye field and prevent inappropriate eye development. Isoforms that carry the homeodomain are required for proper localization of chordotonal organs within the peripheral nervous system and antennal identity; required to activate antennal-specific genes, such as sal and to repress the leg-like expression of dac. Necessary for the nuclear localization of the essential HOX cofactor, extradenticle (exd). Both necessary and sufficient for inner photoreceptors to adopt the polarization-sensitive 'dorsal rim area' (DRA) of the eye fate instead of the color-sensitive default state. This occurs by increasing rhabdomere size and uncoupling R7-R8 communication to allow both cells to express the same opsin rather than different ones as required for color vision.
  • 基因功能參考文獻：
    1. The Ubx cofactor, Homothorax (Hth), was co-enriched with Ubx near enhancers that require Hth, even though Ubx and Hth did not co-localize throughout the nucleus. Thus, microenvironments of high local transcription factor and cofactor concentrations could help low-affinity sites overcome their kinetic inefficiency. PMID: 29095143
    2. the satellite repeats get transcribed in wild type embryos and that this transcription depends on the presence of Hth, which binds to them as well as to the ribosomal DNA region. PMID: 25794008
    3. Functional dissection of the splice variants of the Drosophila gene homothorax (hth PMID: 24075905
    4. Hox genes and homothorax are required for motoneuron survival. PMID: 23536569
    5. Loss of Ct and Hth in the antenna disc resulted in ectopic eye development in the antenna. PMID: 22912416
    6. Data suggest that optimum levels of L and Hth are required to define the boundary between the developing eye and head cuticle on the ventral margin. PMID: 21920354
    7. whole genome ChIP-chip studies to identify all of the Ubx bound regions in the haltere and T3 leg imaginal discs and the sites bound by the Hox cofactor PMID: 21483663
    8. genome-wide analysis of the binding sites for the Hox cofactor Homothorax and the Hox protein Ultrabithorax PMID: 21483667
    9. Regulation of ocellar specification and size by twin of eyeless and homothorax. PMID: 21104743
    10. This work suggests that the transcriptional activity of all members of the Meis/Prep Hth protein family is subject to autoinhibition by their Hth domains, and that the Meis3.2 splice variant encodes a protein that bypasses this autoinhibitory effect. PMID: 20553494
    11. experiments reveal three additional functions for hth: the cell cycle of progenitors is characterized by a relatively long G2 phase, which makes them prone to enter mitosis; hth represses the burst of string/cdc25 expression that precedes G1 arrest PMID: 20036228
    12. homothorax specify antenna fates via regulation of multiple genes. PMID: 11934862
    13. homothorax has a role in leg development in Drosophila PMID: 12385754
    14. homothorax is both necessary and sufficient for inner Photoreceptors to adopt the polarization-sensitive DRA fate instead of the color-sensitive default state PMID: 14636555
    15. Wingless andDecapentaplegic act independently as the primary signals for the repression of teashirt and hth, respectively PMID: 15509768
    16. Alternative splicing of homothorax results in the generation of multiple transcription factors that execute unique functions in vivo. PMID: 16778079
    17. analysis of control of splicing by alternative RNA secondary structures in Dipteran homothorax pre-mRNA PMID: 17114937
    18. In the absence of homothorax gene activity, larval cells in Tr2 become non-proliferative and small imaginal cells are not produced, indicating that homothorax is necessary for proliferation of Tr2 larval cells. PMID: 18455715
    19. Implication of Hth in the regulation of cell growth and endoreplication in larval salivary glands. PMID: 18481089
    20. Homothorax transcription factor is maternally provided and that its function is necessary for the proper assembly of the centric/centromeric heterochromatin during preblastodermic divisions. PMID: 19652544

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    TALE/MEIS homeobox family
  • 組織特異性：
    In the wing disk, the expression is present in the regions corresponding to notum, wing hinge and ventral pleura. In the leg disk, the expression is in the periphery region, corresponding to the proximal segments of the legs. In the antennal disk, the exp
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG17117

    STRING: 7227.FBpp0081732

    UniGene: Dm.2272



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