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  4. Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid (cid)

Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid (cid)

  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid(cid)
  • 貨號：
    CSB-YP893714DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid(cid)
  • 貨號：
    CSB-EP893714DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid(cid)
  • 貨號：
    CSB-EP893714DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid(cid)
  • 貨號：
    CSB-BP893714DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Histone H3-like centromeric protein cid(cid)
  • 貨號：
    CSB-MP893714DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cid
  • Uniprot No.：
  • 別名：
    CENP A; CENP-A homolog; CENPA / CID; CENPA; Centromere identifier; Centromere identifier protein; Centromere protein A; cid; CID/CENP A; CID_DROME; Histone H3 like centromeric protein cid; Histone H3-like centromeric protein cid
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-225
  • 氨基酸序列
    MPRHSRAKRA PRPSANNSKS PNDDDTAFRS PEPEDGTDYG LEFTTSQLTL QDNNRRSSTL RRDAGRRQPA ARDSSTSGEE EDQENRYPTT RSPQTRRMTV QQESKTRAAG PVAAQNQTRR RKAANPMSRA KRMDREIRRL QHHPGTLIPK LPFSRLVREF IVKYSDDEPL RVTEGALLAM QESCEMYLTQ RLADSYMLTK HRNRVTLEVR DMALMAYICD RGRQF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
  • 基因功能參考文獻：
    1. ATP synthase F1 subunits recruited to centromeres by CENP-A are required for male meiosis. PMID: 30006572
    2. centromere assembly factors CAL1 and CENP-C are required for meiotic chromosome segregation, CENP-A assembly and maintenance on sperm, as well as fertility PMID: 27095496
    3. identify two co-evolving regions, CENP-A L1 and the CAL1 N terminus, as critical for lineage-specific CENP-A incorporation PMID: 27093083
    4. A novel role has been described for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster. PMID: 26586808
    5. CENP-A assembly by its loading factor, CAL1, requires RNAPII-mediated transcription of the underlying DNA PMID: 26151904
    6. The amount of Cid that is loaded during each cell cycle appears to be determined primarily by the preexisting centromeric Cid, with little flexibility for compensation of accidental losses. PMID: 23300376
    7. CID nucleosomes are octameric in vivo and that CID dimerization is essential for correct centromere assembly. PMID: 22209075
    8. The F box protein partner of paired (Ppa) mediates CenH3(CID) stability in Drosophila. Ppa depletion results in increased CenH3(CID) levels. Ppa physically interacts with CenH3(CID) through the CATD(CID) that mediates Ppa-dependent CenH3(CID) stability. PMID: 21871803
    9. Drosophila CENP-C is essential for centromere identity. PMID: 20862486
    10. Cid allows for contacts betwen DNA and histones and specific targeting PMID: 12370302
    11. Results suggest that CID mislocalization promotes formation of ectopic centromeres and multicentric chromosomes, which causes chromosome missegregation, aneuploidy, and growth defects. PMID: 16516834
    12. Proteasome-mediated degradation restricts localization of Drosophila CENP-A (CID) to centromeres by eliminating mislocalized CID as well as by regulating available CID levels. PMID: 17090596
    13. Study shows by quantitative fluorescence measurements in living early embryos that functional fluorescent fusion proteins of the Drosophila CENP-A and CENP-C homologs are rapidly incorporated into centromeres during anaphase. PMID: 17222555

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  • 亞細胞定位：
    Nucleus. Chromosome, centromere, kinetochore.
  • 蛋白家族：
    Histone H3 family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG13329

    STRING: 7227.FBpp0086787

    UniGene: Dm.5691



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