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  4. Recombinant Drosophila melanogaster Gram-negative bacteria-binding protein 3 (GNBP3)

Recombinant Drosophila melanogaster Gram-negative bacteria-binding protein 3 (GNBP3)

  • 中文名稱：
    黑腹果蠅GNBP3重組蛋白
  • 貨號：
    CSB-YP885665DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅GNBP3重組蛋白
  • 貨號：
    CSB-EP885665DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅GNBP3重組蛋白
  • 貨號：
    CSB-EP885665DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅GNBP3重組蛋白
  • 貨號：
    CSB-BP885665DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅GNBP3重組蛋白
  • 貨號：
    CSB-MP885665DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GNBP3
  • Uniprot No.：
  • 別名：
    GNBP3; CG5008Gram-negative bacteria-binding protein 3
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    26-490
  • 氨基酸序列
    YEVPK AKIDVFYPKG FEVSIPDEEG ITLFAFHGKL NEEMEGLEAG TWARDIVKAK NGRWTFRDRI TALKPGDTLY YWTYVIYNGL GYREDDGSFV VNGYSGNNAS PHPPVVPVST TPWTPPADPD IDIRLGCTTP KTEVNGAPTR CAGQLVFVDE FNAAKLDPNK WKAERRFSGQ PDYEFNVYVD DAPETLCLAN GHVVLSTNTM KKQFKKGSGE SLDLGEKCTG QANTHDCVRN GRTLNDGLPP MVTAQFSSKD FSFKYGRVEV RAKMPRAQWV TPQIWLQPRR PIYGVDDYRS GQLRIAYTRP NGGNLDLYGA AVLFADEPLR SVKNCLKPGT GNNSEDWSDS FHNYTLEWTP RELRWLVDGK EWCVQGSAKG SFSETTAAGK SLPQAQKLEE GTGLAPFDQE FYLTFGLSVG GFNEYQHEIK PWNERAPQAQ KAFWKEVKKI RDHWLDEGHM KIDYVKVYSL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Involved in the recognition of invading microorganisms. Binds specifically to beta-1,3-glucan and activates the phenoloxidase cascade.
  • 基因功能參考文獻：
    1. Data show that Spn1 is an active protease inhibitor of the serpin superfamily, and support a role of Spn1 as a repressor of GNBP3-dependent Toll activation upon fungal infection. PMID: 21576362
    2. GNBP3 agglutinates fungal cells & activates melanization in a Toll-independent manner. GNBP3 assembles "attack complexes", which comprise phenoloxidase and the necrotic serpin. PMID: 20201042
    3. Here, we report that GNBP3 is a pattern recognition receptor that is required for the detection of fungal cell wall components. Strikingly, we find that there is a second, parallel pathway acting jointly with GNBP3. PMID: 17190605
    4. N-terminal domain of Drosophila Gram-negative binding protein 3 (GNBP3) has a role in beta-glucan recognition PMID: 19692333
    5. GNBP3-N terminal crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 134.79, b = 30.55, c = 51.73 A, beta = 107.4 degrees, and diffracted to 1.7 A using synchrotron radiation. PMID: 19724120

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Insect beta-1,3-glucan binding protein family
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG5008

    STRING: 7227.FBpp0076237

    UniGene: Dm.898



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