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  4. Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 4E (eIF-4E)

Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 4E (eIF-4E)

  • 中文名稱：
    黑腹果蠅eIF-4E重組蛋白
  • 貨號：
    CSB-YP007556DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF-4E重組蛋白
  • 貨號：
    CSB-EP007556DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF-4E重組蛋白
  • 貨號：
    CSB-EP007556DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅eIF-4E重組蛋白
  • 貨號：
    CSB-BP007556DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF-4E重組蛋白
  • 貨號：
    CSB-MP007556DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eIF-4E
  • Uniprot No.：
  • 別名：
    eIF4E1; eIF-4E; Eif4e; CG4035Eukaryotic translation initiation factor 4E1; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-259
  • 氨基酸序列
    MQSDFHRMKN FANPKSMFKT SAPSTEQGRP EPPTSAAAPA EAKDVKPKED PQETGEPAGN TATTTAPAGD DAVRTEHLYK HPLMNVWTLW YLENDRSKSW EDMQNEITSF DTVEDFWSLY NHIKPPSEIK LGSDYSLFKK NIRPMWEDAA NKQGGRWVIT LNKSSKTDLD NLWLDVLLCL IGEAFDHSDQ ICGAVINIRG KSNKISIWTA DGNNEEAALE IGHKLRDALR LGRNNSLQYQ LHKDTMVKQG SNVKSIYTL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In 0-1 hour embryos, forms a complex with me31B, cup, tral and pAbp which binds to various mRNAs including maternal mRNAs, and downregulates their expression during the maternal-to-zygotic transition.
  • 基因功能參考文獻：
    1. Both eIF4E-1 and eIF4E-3 are required in spermatocytes for chromosome condensation and cytokinesis during the meiotic stages. PMID: 25849588
    2. Protein-protein interactions rather than interactions with the mRNA are essential for the recruitment of eIF4E and for a putative nucleation function. PMID: 22507384
    3. Eukaryotic initiation factor 4E-3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila. PMID: 22833128
    4. eIF4E regulates the sex-specific expression of the master switch gene Sxl. PMID: 21829374
  • 亞細胞定位：
    Cytoplasm, Cytoplasmic ribonucleoprotein granule.
  • 蛋白家族：
    Eukaryotic initiation factor 4E family
  • 組織特異性：
    Expressed at the posterior end of developing oocytes (at protein level). Preferential expression in the pole cells, at different developmental stages.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4035

    STRING: 7227.FBpp0076215

    UniGene: Dm.7012



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