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  4. Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 3 subunit E (eIF3-S6)

Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 3 subunit E (eIF3-S6)

  • 中文名稱：
    黑腹果蠅eIF3e重組蛋白
  • 貨號：
    CSB-YP007534DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF3e重組蛋白
  • 貨號：
    CSB-EP007534DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF3e重組蛋白
  • 貨號：
    CSB-EP007534DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅eIF3e重組蛋白
  • 貨號：
    CSB-BP007534DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅eIF3e重組蛋白
  • 貨號：
    CSB-MP007534DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eIF3e
  • Uniprot No.：
  • 別名：
    eIF3e; eIF-3p48; eIF3-S6; Int6; CG9677; Eukaryotic translation initiation factor 3 subunit E; eIF3e; Eukaryotic translation initiation factor 3 subunit 6
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-435
  • 氨基酸序列
    MANFDLTRIN CQFLDRHLTF PLLEFLCGKE IYNQQELLEY ILETVNKTNM IDYTMDTRKR LNLSQEMPEE LVQRKAEVLA TLKQLQNEVA PIMKATDILK NGESMKDSKT FVNALQKDYN FKVEHLESAY KLAKYLYECG NYQESTSYLY FCLIVMSPND KNYLNVLWGK LAAEILTLNW NTALEDLTRL RDYIDNANFS TIQALQQRTW LIHWSVLVFF NHPKGRDLII EMFLYKPLYL NAIQTMCPHI MRYLATAVVI NRTRRNALKD LIKVIQQESY TYRDPITEFL ECLYVNFDFE GARLKLHECQ TVILNDFFIV ACLNEFVEDA RLMIFETFCR IHQCITISML ADKLNMKPNE AECWIVNLIR NARLNAKIDS KLGHVVMGTQ PLSPYQQLVE KIDSLSMRSE HLAGLIERKS KQKQNQESAD SWKYY
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation. During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression. This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends. Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5.
  • 基因功能參考文獻(xiàn)：
    1. Depletion of Int6 results in short mitotic spindles and deformed centromeres and kinetochores. Int6-mediated ubiquitination is required to control the activity of Klp67A. PMID: 28505193
    2. Int6 and the COP9 signalosome play opposing roles in regulation of cullin neddylation PMID: 18493598
  • 亞細(xì)胞定位：
    Cytoplasm. Microsome. Endoplasmic reticulum.
  • 蛋白家族：
    EIF-3 subunit E family
  • 組織特異性：
    Expression levels in females and males are relatively similar 10 days after oviposition, however by day 15 expression is higher in gravid females than in males (at protein level).
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG9677

    STRING: 7227.FBpp0075104

    UniGene: Dm.3105



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