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  4. Recombinant Drosophila melanogaster Dynamin (shi), partial

Recombinant Drosophila melanogaster Dynamin (shi), partial

  • 中文名稱：
    黑腹果蠅shi重組蛋白
  • 貨號：
    CSB-YP326735DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅shi重組蛋白
  • 貨號：
    CSB-EP326735DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅shi重組蛋白
  • 貨號：
    CSB-EP326735DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅shi重組蛋白
  • 貨號：
    CSB-BP326735DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅shi重組蛋白
  • 貨號：
    CSB-MP326735DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    shi
  • Uniprot No.：
  • 別名：
    shi; CG18102Dynamin; EC 3.6.5.5; Protein shibire; dDyn
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Microtubule-associated force-producing protein which is involved in the production of microtubule bundles and which is able to bind and hydrolyze GTP. Implicated in endocytic protein sorting.
  • 基因功能參考文獻：
    1. results implicate mutant dynamin as an effective seizure suppressor, suggesting that targeting or limiting the availability of synaptic vesicles could be an effective and general method of controlling epilepsy disorders PMID: 26341658
    2. These studies identify novel morphogenetic roles (i.e., tube closure and biased apical expansion), and expand upon established roles (i.e., cell intercalation and adhesion remodeling), for Dynamin in tubulogenesis. PMID: 26542010
    3. Dynamin is critically involved in the stabilization of Clathrin- and AP2-dependent endocytic pits. PMID: 24662566
    4. The shibire gene product, Dynamin, appears to be required for an immediate early response to ethanol that triggers a cellular response leading to rapid functional tolerance. PMID: 21797886
    5. A temperature sensitive shibire mutant was used to trap synaptic vesicles at the plasma membrane and extracted sterols from synaptic vesicles. PMID: 21106824
    6. Mutation in shibire causes male-male courtship behavior due to disruption of synaptic transmission PMID: 12239352
    7. The shi(ts1) mutation induces a conformational change, involving the GTP binding site, that affects dynamin aggregation. PMID: 12382260
    8. Expression of a temperature-sensitive shibire (shi) transgene in a subset of alpha/beta neurons resulted in odor-pair-specific disruption of learning. PMID: 16980542
    9. DRP mutants affect synaptic transmission in a manner similar to the Drosophila sesBorg mutants. Mitochondria with abnormal morphology in DRP mutants might lead to a deficit in ATP levels or lowered rates of ATP repletion at the synapse. PMID: 17233640

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  • 亞細胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG18102

    STRING: 7227.FBpp0305866

    UniGene: Dm.3444



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