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  4. Recombinant Drosophila melanogaster Dual specificity protein phosphatase Mpk3 (Mkp3)

Recombinant Drosophila melanogaster Dual specificity protein phosphatase Mpk3 (Mkp3)

  • 中文名稱：
    黑腹果蠅Mkp3重組蛋白
  • 貨號：
    CSB-YP893338DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅Mkp3重組蛋白
  • 貨號：
    CSB-EP893338DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅Mkp3重組蛋白
  • 貨號：
    CSB-EP893338DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅Mkp3重組蛋白
  • 貨號：
    CSB-BP893338DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅Mkp3重組蛋白
  • 貨號：
    CSB-MP893338DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Mkp3
  • Uniprot No.：
  • 別名：
    Mkp3; CG14080Dual specificity protein phosphatase Mpk3; EC 3.1.3.16; EC 3.1.3.48; Drosophila MKP3; DMKP3; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-411
  • 氨基酸序列
    MPETEHETCS KEWLQSQLRS LDSKDLILLD CRGSHEYSES HIRGAVNLCI PSIVLRRLAV GKIDLASTIK SPELKQRIQS GYKLCWFILY NGEGVPGQNQ EIAGAGSLAV AMDSIISILH RRLKQDGCRV VALQDGFNNF RQAFPEWCED DNQTHSKEIE SSRNVQTDQL MGLRSLRIST TQSDSACSSS AESSDCESSS HHHHHHSHHN YNEAPVEIIP GLLFLGNATH SCDSEALKKY NIKYVLNVTP DLPNKFKESG DIKYLQIPIT DHYSQDLAIH FPDAIQFIEE ARSASSVVLV HCLAGVSRSV TVTLAYLMHT RGLSLNDAFA MVRDRKPDVS PNFHFMQQLL SFESQLRLRP GSRFSCSCIA PDCNCMQTTG FMATHLANAT GVSPDSGIEF DRWTPSDTGL K
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Negatively regulates the activity of members of the MAP kinase family in response to changes in the cellular environment. Has a specificity for the ERK family. Acts as negative regulator in a variety of developmental processes including cell differentiation and proliferation controlled by the Ras/ERK pathway. Suppresses the photoreceptor cell differentiation and wing vein formation. Required for proper oogenesis and early embryogenesis. Functions autonomously in a subset of photoreceptor progenitor cells in eye imaginal disks. Appears also to be required in surrounding non-neuronal cells for ommatidial patterning and photoreceptor differentiation. Plays a role in the maintenance of epithelial integrity during tracheal development.
  • 基因功能參考文獻：
    1. We show that Tay bridge antagonizes EGFR signalling in the Drosophila melanogaster wing disc and other tissues, and that the protein interacts with both Erk and Mkp3 PMID: 24348264
    2. MKP-3 is critically involved in cell differentiation, proliferation, and gene expression by suppressing the Ras/ERK pathway, specifically binding to ERK via the N-terminal ERK-binding domain of MKP-3. PMID: 14701731
    3. Drosophila ERK specifically regulates DMKP-3 in terms of its enzyme activity and protein stability esssential for regulation. PMID: 15033693
    4. Study suggests that Mkp3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway. PMID: 15704110
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
  • 組織特異性：
    Ubiquitous expression in eye and wing imaginal disks. Enriched in ovary.
  • 數據庫鏈接：

    KEGG: dme:Dmel_CG14080

    STRING: 7227.FBpp0303122

    UniGene: Dm.3967



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