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Recombinant Drosophila melanogaster Dorsal-related immunity factor Dif (Dif), partial

  • 中文名稱:
    黑腹果蠅Dif重組蛋白
  • 貨號:
    CSB-YP310253DLU
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    黑腹果蠅Dif重組蛋白
  • 貨號:
    CSB-EP310253DLU
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    黑腹果蠅Dif重組蛋白
  • 貨號:
    CSB-EP310253DLU-B
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    黑腹果蠅Dif重組蛋白
  • 貨號:
    CSB-BP310253DLU
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    黑腹果蠅Dif重組蛋白
  • 貨號:
    CSB-MP310253DLU
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    Dif; CG6794Dorsal-related immunity factor Dif
  • 種屬:
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Mediates an immune response in larvae. DIF binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of the immunity gene CECA1. Involved in the melanization immune response to bacterial challenge, possibly by acting in a Toll signaling pathway that downregulates Spn27A in response to infection. Part of a signaling pathway involving NF-kappa-B (rel) and Toll-related receptors, that functions in the apoptosis of unfit cells during cell competition. May be part of a NF-kappa-B and Tollo signaling cascade that regulates development of the peripheral nervous system.
  • 基因功能參考文獻:
    1. These results, not only revealed a novel function and modulation pattern of miR-958, but also provided a new insight into the underlying molecular mechanisms of Toll signaling in regulation of innate immunity. PMID: 27974298
    2. Fasting increases survival to cold in dif mutant Drosophila melanogaster. PMID: 25663303
    3. DIF factor seems to be involved in the response to fungal infection after a mild cold stress and in the resistance to heat. DIF seems to have no role in the increased longevity of non-infected flies and resistance to a severe cold shock. PMID: 22791143
    4. Data show that the DIF and Relish complex is detectable in whole animal extracts, suggesting that this heterodimer may function in vivo to increase the spectrum and level of antimicrobial peptide production in response to different infections. PMID: 20679214
    5. Various fungi stimulated antimicrobial peptides through at least two different pathways requiring Relish and/or Dif. PMID: 14985331
    6. Dif and Dorsal control crucial aspects of the cellular immune response PMID: 17060622
    7. Molecular modeling was used to compare the tertiary structure of Dif with two other Drosophila proteins. PMID: 17785540

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  • 亞細胞定位:
    Cytoplasm. Nucleus.
  • 數(shù)據(jù)庫鏈接:

    KEGG: dme:Dmel_CG6794

    STRING: 7227.FBpp0290792

    UniGene: Dm.19845



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