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  4. Recombinant Drosophila melanogaster Deoxyhypusine hydroxylase (nero)

Recombinant Drosophila melanogaster Deoxyhypusine hydroxylase (nero)

  • 中文名稱：
    黑腹果蠅nero重組蛋白
  • 貨號：
    CSB-YP892698DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅nero重組蛋白
  • 貨號：
    CSB-EP892698DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅nero重組蛋白
  • 貨號：
    CSB-EP892698DLU-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅nero重組蛋白
  • 貨號：
    CSB-BP892698DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅nero重組蛋白
  • 貨號：
    CSB-MP892698DLU
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    nero
  • Uniprot No.：
  • 別名：
    nero; l(3)s1921; CG2245Deoxyhypusine hydroxylase; DOHH; EC 1.14.99.29; Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-302
  • 氨基酸序列
    MVSQQQIEAI GGVLNNKERP LKERFRALFT LKNIGGGAAI EAISKAFDDD SALLKHELAY CLGQMQDAQA LDILTKVLKD TTQEPMVRHE AAEAMGAIGH PDVLPILEEY KQDPVVEVAE TCAIALDRVR WLQSGQKVDD SNPYASVDPS PPTAGDKSVT ELKAIYLDAQ QSLFDRYRAM FSLRNLRTEE SVLAIAEGLK DSSALFRHEV AFVLGQLQEP CSIPFLQENL EDRLENEMVR HECAEALGAI ATEDCIQILN RYAEDDKRVV KESCVIALDM CEYENSPEFQ YADGLAKLDA TK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor. Essential for organismal viability and plays a role in a wide number of important processes such as cell growth and proliferation, and regulates induction of autophagy and protein synthesis. Has a role in eIF-5A-mediated translational control.
  • 基因功能參考文獻：
    1. Results describe the identification and characterization of nero (CG2245), the Drosophila melanogaster deoxyhypusine hydroxylase (DOHH) homologue, and indicate that nero and eIF5A are required for cell growth and affect autophagy and protein synthesis. PMID: 19546244
  • 亞細胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In larval imaginal disk and Garland cells.
  • 蛋白家族：
    Deoxyhypusine hydroxylase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG2245

    STRING: 7227.FBpp0085222

    UniGene: Dm.29814



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