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  4. Recombinant Drosophila melanogaster Cell death protein W (W)

Recombinant Drosophila melanogaster Cell death protein W (W)

  • 中文名稱：
    黑腹果蠅hid重組蛋白
  • 貨號：
    CSB-YP632574DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅hid重組蛋白
  • 貨號：
    CSB-EP632574DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅hid重組蛋白
  • 貨號：
    CSB-EP632574DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅hid重組蛋白
  • 貨號：
    CSB-BP632574DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅hid重組蛋白
  • 貨號：
    CSB-MP632574DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hid
  • Uniprot No.：
  • 別名：
    hid; W; CG5123; Cell death protein hid; Protein head involution defective; Protein wrinkled
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-410
  • 氨基酸序列
    MAVPFYLPEG GADDVASSSS GASGNSSPHN HPLPSSASSS VSSSGVSSAS ASSASSSSSA SSDGASSAAS QSPNTTTSSA TQTPMQSPLP TDQVLYALYE WVRMYQSQQS APQIFQYPPP SPSCNFTGGD VFFPHGHPNP NSNPHPRTPR TSVSFSSGEE YNFFRQQQPQ PHPSYPAPST PQPMPPQSAP PMHCSHSYPQ QSAHMMPHHS APFGMGGTYY AGYTPPPTPN TASAGTSSSS AAFGWHGHPH SPFTSTSTPL SAPVAPKMRL QRSQSDAARR KRLTSTGEDE REYQSDHEAT WDEFGDRYDN FTAGRERLQE FNGRIPPRKK KSSNSHSSSS NNPVCHTDSQ PGGTSQAESG AIHGHISQQR QVERERQKAK AEKKKPQSFT WPTVVTVFVL AMGCGFFAAR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Activator of apoptosis, with grim and rpr, that acts on the effector Dredd. Seems to act genetically upstream of baculoviral anti-apoptotic p35. Blocks Diap2 from binding and inactivating the effector caspase Drice. Might regulate apoptosis downstream of Clbn/NEMF.
  • 基因功能參考文獻：
    1. hid is required during pupariation for caspase-dependent pruning of class IV da neurons and Nos and Pum delay pruning. PMID: 27256879
    2. loss of function mutants shows persisting wing epithelial cells and, furthermore, silencing of the hormone bursicon in the CNS produced collective cell death defective phenotypes manifested in the wing epithelium PMID: 26226435
    3. Study provide evidence that miR-6/11 functions through regulation of the proapoptotic genes, reaper (rpr), head involution defective (hid), grim and sickle (skl). PMID: 22095284
    4. head involution defect protein and transcripts accumulate specifically in polar cells destined to die PMID: 21113144
    5. FoxO targets hid and bim in Drosophila and human, respectively, are responsible for the LRRK2/dLRRK-mediated cell death. PMID: 20624856
    6. Rpr is able to self-associate and also binds to Hid and Grim. PMID: 20837774
    7. In the Drosophila eye, Ras may protect cells from apoptosis by inhibiting both JNK and Hid activities. PMID: 19840402
    8. hid mediates programmed cell death during drosophila development PMID: 11853767
    9. Hid, Rpr and Grim negatively regulate DIAP1 levels through distinct mechanisms. PMID: 12021767
    10. Drosophila Bruce can potently suppress Rpr- and Grim-dependent but not Hid-dependent cell death PMID: 12121627
    11. head involution defective (Hid)-triggered apoptosis requires caspase-8 but not FADD (Fas-associated death domain) and is regulated by Erk in mammalian cells PMID: 12122017
    12. co-expression of Op-IAP3 and the pro-apoptotic Drosophila protein HID resulted in the ubiquitination of HID. PMID: 15325084
    13. Both Grim and Hid were demonstrated to interact with Amsacta moorei Entomopoxvirus inhibitor of apoptosis (AMV-IAP) in vivo, and Grim- or Hid-induced cell death was suppressed when AMV-IAP was coexpressed. PMID: 15731262
    14. Hid is located downstream of LKB1 and is invloved in LKB1 sependent apoptosis. PMID: 16273080
    15. These results show that internal ribosome entry sites-dependent initiation may play a role in the translation of Drosophila proapoptotic genes and suggest a variety of regulatory pathways. PMID: 16794603
    16. germ line is maintained through the regulated expression of RHG genes PMID: 17449640
    17. Study examined the interactions of the bantam miRNA with the 3' UTR of the hid mRNA and found the potential contribution of the 3-9 seed register to microRNA-mediated gene regulation. PMID: 19286629
    18. pro-apoptotic gene hid plays an important role in the execution of cell death in Drosophila. (Review) PMID: 19554451

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  • 數據庫鏈接：

    KEGG: dme:Dmel_CG5123

    STRING: 7227.FBpp0074899

    UniGene: Dm.5624



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