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  4. Recombinant Drosophila melanogaster Cell death protein Grim (grim)

Recombinant Drosophila melanogaster Cell death protein Grim (grim)

  • 中文名稱：
    黑腹果蠅grim重組蛋白
  • 貨號：
    CSB-YP632804DLU
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅grim重組蛋白
  • 貨號：
    CSB-EP632804DLU
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅grim重組蛋白
  • 貨號：
    CSB-EP632804DLU-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅grim重組蛋白
  • 貨號：
    CSB-BP632804DLU
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅grim重組蛋白
  • 貨號：
    CSB-MP632804DLU
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    grim
  • Uniprot No.：
  • 別名：
    grim; CG4345Cell death protein Grim
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-138
  • 氨基酸序列
    MAIAYFIPDQ AQLLARSYQQ NGQQTAASPR TTATAAAPSQ QQQQSQQQQQ QQRHHHQQQR PQFRANISVP LGSQQGSMTM SEFGCWDLLA QIFCYALRIY SYSSSQRQPT VIQISFEISS GGQNNDEDDV TDATSKEN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Activator of apoptosis, independent of rpr and hid, that acts on the effector Dredd.
  • 基因功能參考文獻：
    1. findings demonstrate that neuroblast apoptosis during embryogenesis requires the coordinated expression of the cell death genes grim and reaper, and possibly sickle PMID: 21558369
    2. Programmed cell death of microchaete glial cells represents an exceptional opportunity to study the mitochondrial proapoptotic process induced by Grim. PMID: 20558283
    3. When Grim is overexpressed in vivo, both the N-terminal & GH3 domains are equally necessary & cooperate for apoptosis induction. The N-terminal and GH3 Grim domains activate independent apoptotic pathways that synergize to induce programmed cell death. PMID: 12093734
    4. Analysis of a Grim mutant that promoted XIAP degradation PMID: 14570909
    5. role in countering DrICE inhibition by DIAP1 PMID: 15107838
    6. Grim is located downstream of LKB1 and is invloved in LKB1 sependent apoptosis. PMID: 16273080
    7. temperature shifts and dietary restriction, which slow the rate of aging in D. melanogaster, extend the window of neuronal susceptibility to GRIM over-expression in a way that scales to lifespan. PMID: 19428445

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  • 數據庫鏈接：

    KEGG: dme:Dmel_CG4345

    STRING: 7227.FBpp0074896

    UniGene: Dm.2372



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