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  4. Recombinant Drosophila melanogaster Actin-binding protein anillin (scra), partial

Recombinant Drosophila melanogaster Actin-binding protein anillin (scra), partial

  • 中文名稱(chēng)：
    黑腹果蠅scra重組蛋白
  • 貨號(hào)：
    CSB-YP895097DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅scra重組蛋白
  • 貨號(hào)：
    CSB-EP895097DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅scra重組蛋白
  • 貨號(hào)：
    CSB-EP895097DLU-B
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅scra重組蛋白
  • 貨號(hào)：
    CSB-BP895097DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    黑腹果蠅scra重組蛋白
  • 貨號(hào)：
    CSB-MP895097DLU
  • 說(shuō)明書(shū)：
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    scra
  • Uniprot No.：
  • 別名：
    scra; ani; CG2092Anillin; Actin-binding protein 8; ABP8; Protein scraps
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression and proper formation of the midbody. Required during cellularization of syncytial embryos for the proper formation and function of the furrow canals, the stable inward folds of the plasma membrane which separate the peripheral nuclei. Also required for the formation of the pole cells, the progenitors of the adult germline which are formed by cytokinesis of the cytoplasmic buds at the posterior pole of the syncytial embryo. Essential for embryonic viability.
  • 基因功能參考文獻(xiàn)：
    1. we show that the transition from the actomyosin contractile ring to the midbody ring proceeds via a previously uncharacterized maturation process that requires opposing mechanisms of removal and retention of the scaffold protein Anillin. PMID: 24217622
    2. Anillin is required for complete closure of the contractile ring and formation of the midbody ring during cytokinesis. PMID: 22226749
    3. the anillin pleckstrin homology domain has two functions: targeting anillin to the plasma membrane furrow by binding to phosphatidylinositol phosphate lipids to maintain furrow organization and recruiting septins to the furrow. PMID: 22197245
    4. Data suggest that Cindr and Anillin cooperate to promote intercellular bridge stability during incomplete cytokinesis in Drosophila melanogaster. PMID: 20451383
    5. Results show that the anillin-septin and cadherin-catenin complexes can serve as alternative cassettes to promote tight physical coupling of F-actin and myosin II to the cleavage furrow and successful completion of cytokinesis. PMID: 20237160
    6. anillin has a role in spatially regulating the contractile activity of myosin II during cytokinesis PMID: 15496454
    7. important role for scraps in scaffolding cleavage furrow components, directly stabilizing intracellular bridges PMID: 15930114
    8. interaction with cleavage furrow mediates RacGAP50C's control of actomyosin ring assembly PMID: 18349071
    9. anillin and Peanut are involved in pseudocleavage furrow ingression in syncytial embryos, a process that is regulated by Ran PMID: 18579688

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. Accumulates in the nucleus of newly divided cells in a diffuse staining pattern, thereby restarting the cycle.
  • 組織特異性：
    Accumulates in the ring canals that interconnect cells of the germline cysts in males and the ovarian follicles in females. These structures develop from arrested contractile rings after a specialized cytokinesis in which the closing of the invaginating p
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: dme:Dmel_CG2092

    STRING: 7227.FBpp0087986

    UniGene: Dm.508



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