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  4. Recombinant Dog Cardiac phospholamban (PLN), partial

Recombinant Dog Cardiac phospholamban (PLN), partial

  • 中文名稱：
    熟悉的犬PLN重組蛋白
  • 貨號：
    CSB-YP018198DO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    熟悉的犬PLN重組蛋白
  • 貨號：
    CSB-EP018198DO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    熟悉的犬PLN重組蛋白
  • 貨號：
    CSB-EP018198DO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    熟悉的犬PLN重組蛋白
  • 貨號：
    CSB-BP018198DO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    熟悉的犬PLN重組蛋白
  • 貨號：
    CSB-MP018198DO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PLN; Cardiac phospholamban; PLB
  • 種屬：
    Canis familiaris (Dog) (Canis lupus familiaris)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 基因功能參考文獻：
    1. PLB mRNA was reduced 16-fold, and PLB protein was reduced 5-fold. PMID: 21542669
    2. PLB decreases Ca(2+) binding to SERCA2a by stabilizing a unique E2.ATP state that is unable to bind thapsigargin or vanadate. PMID: 20622261
    3. Ca2+ binding to site I of the cardiac Ca2+ pump is sufficient to dissociate phospholamban PMID: 19948724
    4. phospholamban leucine 31 has roles in structural and functional interactions with SERCA2a PMID: 15644311
    5. Phospholamban (PLB) oligomerization, quaternary structure, and sarco(endo)plasmic reticulum calcium ATPase (SERCA) binding were quantified by fluorescence resonance energy transfer (FRET) in an intact cellular environment. PMID: 18287099
    6. Activation of cardiac muscle sarcoplasmic reticulum calcium-transporting ATPase (SERCA2a) dephosphorylation by nitroxyl (HNO) is PLN-dependent; PLN thiols are targets for HNO. PMID: 19053265

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Phospholamban family
  • 組織特異性：
    Heart.
  • 數據庫鏈接：


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