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  4. Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 2 subunit 3 (eif2s3)

Recombinant Dictyostelium discoideum Eukaryotic translation initiation factor 2 subunit 3 (eif2s3)

  • 中文名稱：
    盤基網柄菌eif2s3重組蛋白
  • 貨號：
    CSB-YP706902DKK
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    盤基網柄菌eif2s3重組蛋白
  • 貨號：
    CSB-EP706902DKK
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    盤基網柄菌eif2s3重組蛋白
  • 貨號：
    CSB-EP706902DKK-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    盤基網柄菌eif2s3重組蛋白
  • 貨號：
    CSB-BP706902DKK
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    盤基網柄菌eif2s3重組蛋白
  • 貨號：
    CSB-MP706902DKK
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eif2s3
  • Uniprot No.：
  • 別名：
    eif2s3; eif2G; DDB_G0278967Eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit gamma; eIF-2-gamma
  • 種屬：
    Dictyostelium discoideum (Slime mold)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-460
  • 氨基酸序列
    MATKNKPNLS VQDVKLLNVN ELTPLTPNVI SRQATINIGT IGHVAHGKST VVKAISGVLT VRFTSEFKRN ITIKLGYANA KIYKCDNPQC ERPGCYKSAR SNTEDNPQCE RPGCGGRMTL LRHVSFVDCP GHDVLMATML NGAAVMDAAL LLIAGNESCP QPQTSEHIAA IEIMNLKNII ILQNKIDLVK EAAAQEQYGQ ILKFIQGTIA ENAPIIPISA QMKYNIDVIC EYIVKKIPIP VRDFTSDPRM IVIRSFDVNK PGSRVDEIKG GVAGGSILKG VLKIGDEIEV RPGVISKELD GKIKCSPIFC RIISLFAEEN ELQYAVPGGL IGVGTKIDPT LCRADRLVGQ VLGSVGKLPE IFVALEVNFF LLRRLLGVKS DGDKQSKVKK LSKEDTLMVN IGSTSTGCRV VAVKHDLAKL QLLTPVCSQE GEKIALSRRV DKNWRLIGWG EIKKGTVLTN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B.
  • 蛋白家族：
    TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EIF2G subfamily
  • 數據庫鏈接：


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