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  4. Recombinant Danio rerio Sphingosine 1-phosphate receptor 2 (s1pr2), partial

Recombinant Danio rerio Sphingosine 1-phosphate receptor 2 (s1pr2), partial

  • 中文名稱：
    斑馬魚s1pr2重組蛋白
  • 貨號：
    CSB-YP884843DIL1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    斑馬魚s1pr2重組蛋白
  • 貨號：
    CSB-EP884843DIL1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    斑馬魚s1pr2重組蛋白
  • 貨號：
    CSB-EP884843DIL1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    斑馬魚s1pr2重組蛋白
  • 貨號：
    CSB-BP884843DIL1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    斑馬魚s1pr2重組蛋白
  • 貨號：
    CSB-MP884843DIL1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    s1pr2; edg5; Sphingosine 1-phosphate receptor 2; S1P receptor 2; S1P2; Sphingosine 1-phosphate receptor Edg-5; S1P receptor Edg-5
  • 種屬：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P receptor is critical for cell migration and epithelial integrity during vertebrate embryogenesis. Receptor for the chemokine-like protein FAM19A5. Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration.
  • 基因功能參考文獻：
    1. In embryos defective for S1pr2/Galpha13 signaling, endocardial precursors failed to migrate towards the midline, and the presumptive endocardium surrounded the bilaterally-located myocardial cells rather than being encompassed by them. PMID: 27158029
    2. beta-arrestin 2 and GRK2 colocalize with S1p2 in developing zebrafish embryos and depletion of GRK2 in the S1p2 R150H miles apart zebrafish partially rescued cardia bifida. PMID: 25555130
    3. S1pr2/Galpha13 signaling controls myocardial migration by regulating endoderm convergence. PMID: 23318642
    4. S1P(2) loss in a zebrafish mutant disrupted epithelial cell extrusion. PMID: 21555463
    5. mil encodes Edg5, a sphingosine-1-phosphate receptor. PMID: 18701549

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  • 相關疾病：
    Defects in s1pr2 are a cause of heart development abnormality named miles apart (mil). In all vertebrates, the myocardial progenitors involute early during gastrulation and come to occupy bilateral positions in the anterior lateral plate mesoderm (LPM). During somitogenesis, these cells undergo a second phase of migration toward the midline and fuse to form the definitive heart tube. Defects in S1PR2 disrupt this process, leading to the formation of two laterally positioned hearts (cardia bifida). The mil phenotype for which two recessive alleles exist, mil(m93) and mil(te273) are fully penetrant. Mil(m93)/mil(te273) transheterozygous embryos display the same phenotype as homozygotes for either single mutant allele. In addition to cardia bifida, mil mutants display epithelial tail blisters, indicative of a defect in epithelial integrity.
  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 數據庫鏈接：


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