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  4. Recombinant Cysteine protease-like virA (virA)

Recombinant Cysteine protease-like virA (virA)

  • 中文名稱：
    弗累克斯訥氏桿菌virA重組蛋白
  • 貨號：
    CSB-YP762461SZB
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌virA重組蛋白
  • 貨號：
    CSB-EP762461SZB-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌virA重組蛋白
  • 貨號：
    CSB-BP762461SZB
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    弗累克斯訥氏桿菌virA重組蛋白
  • 貨號：
    CSB-MP762461SZB
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    virA
  • Uniprot No.：
  • 別名：
    virA; CP0181; pWR501_0191; Cysteine protease-like VirA; EC 3.4.22.-; Effector protein VirA
  • 種屬：
    Shigella flexneri
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-400
  • 氨基酸序列
    MQTSNITNHE RNDSSWMSTV KSTTEVSWNK LSFCDILLKI ITFGIYSPHE TLAEKHSEKK LMDSFSPSLS QDKMDGEFAH ANIDGISIRL CLNKGICSVF YLDGDKIQST QLSSKEYNNL LSSLPPKQFN LGKVHTITAP VSGNFKTHKP APEVIETAIN CCTSIIPNDD YFHVKDTDFN SVWHDIYRDI RASDSNSTKI YFNNIEIPLK LIADLINELG INEFIDSKKE LQMLSYNQVN KIINSNFPQQ DLCFQTEKLL FTSLFQDPAF ISALTSAFWQ SLHITSSSVE HIYAQIMSEN IENRLNFMPE QRVINNCGHI IKINAVVPKN DTAISASGGR AYEVSSSILP SHITCNGVGI NKIETSYLVH AGTLPSSEGL RNAIPPESRQ VSFAIISPDV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.
  • 基因功能參考文獻：
    1. Study discovers that bacterial effectors including VirA from nonvacuole Shigella flexneri and EspG from extracellular Enteropathogenic Escherichia coli (EPEC) harbor TBC-like dual-finger motifs and exhibits potent RabGAP activities. Specific inactivation of Rab1 by VirA/EspG disrupts ER-to-Golgi trafficking. PMID: 22939626
  • 亞細胞定位：
    Secreted. Note=Translocated into the host cell via the type III secretion system (TTSS). Localizes in the cytoplasm of the infected cell.
  • 蛋白家族：
    Protease EspG/VirA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: sfl:CP0181



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