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  4. Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta (grdE)

Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta (grdE)

  • 中文名稱：
    Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta(grdE)
  • 貨號：
    CSB-YP863684DUS
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta(grdE)
  • 貨號：
    CSB-EP863684DUS
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta(grdE)
  • 貨號：
    CSB-EP863684DUS-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta(grdE)
  • 貨號：
    CSB-BP863684DUS
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Clostridium sticklandii Glycine reductase complex component B subunits alpha and beta(grdE)
  • 貨號：
    CSB-MP863684DUS
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    grdE
  • Uniprot No.：
  • 別名：
    grdE; CLOST_1110; Glycine reductase complex component B subunits alpha and beta; EC 1.21.4.2; Selenoprotein PB alpha/beta) [Cleaved into: Betaine reductase component B subunit beta; Betaine reductase component B subunit alpha]
  • 種屬：
    Clostridium sticklandii (strain ATCC 12662 / DSM 519 / JCM 1433 / NCIB 10654)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-241
  • 氨基酸序列
    MRLEVGNIFI KDIQFGDSTK VENGVLYVNK QELISELSSD EHIKSIDMEI VRPGESVRIA PVKDVIEPRV KVEGNGGIFP GFLSKVDTVG EGKTNVLKGA AVVTTGKVVG FQEGIIDMTG PGADYTPFSK TCNVVIIAEP VDGLKQHDHE AALRMVGLKA GKYLGEAGRN ITPDEVKVYE TKPIFESVKE YPNLPKVAYV YMLQTQGLLH DTYVYGVDAK KIIPTLIYPT EVMDGAILSG N
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.
  • 數據庫鏈接：


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