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  4. Recombinant Chicken Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PCK1), partial

Recombinant Chicken Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (PCK1), partial

  • 中文名稱：
    雞PCK1重組蛋白
  • 貨號：
    CSB-YP017613CH
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    雞PCK1重組蛋白
  • 貨號：
    CSB-EP017613CH
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    雞PCK1重組蛋白
  • 貨號：
    CSB-EP017613CH-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    雞PCK1重組蛋白
  • 貨號：
    CSB-BP017613CH
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    雞PCK1重組蛋白
  • 貨號：
    CSB-MP017613CH
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PCK1; Phosphoenolpyruvate carboxykinase; cytosolic [GTP]; PEPCK-C; EC 4.1.1.32
  • 種屬：
    Gallus gallus (Chicken)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle. At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor.
  • 基因功能參考文獻：
    1. androgen status negatively regulated the PCK1 gene in male chickens. PMID: 23544081
    2. analysis of restriction fragment length and single strand conformational polymorphisms in chicken mitochondrial phosphoenol-pyruate carboxykinase gene and its association with egg production PMID: 19090282
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Phosphoenolpyruvate carboxykinase [GTP] family
  • 數據庫鏈接：


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