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  4. Recombinant Chicken Gap junction alpha-8 protein (GJA8), partial

Recombinant Chicken Gap junction alpha-8 protein (GJA8), partial

  • 中文名稱：
    雞GJA8重組蛋白
  • 貨號：
    CSB-YP009449CH1
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    雞GJA8重組蛋白
  • 貨號：
    CSB-EP009449CH1
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    雞GJA8重組蛋白
  • 貨號：
    CSB-EP009449CH1-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    雞GJA8重組蛋白
  • 貨號：
    CSB-BP009449CH1
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    雞GJA8重組蛋白
  • 貨號：
    CSB-MP009449CH1
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GJA8
  • Uniprot No.：
  • 別名：
    GJA8; Gap junction alpha-8 protein; Connexin-45.6; Cx45.6
  • 種屬：
    Gallus gallus (Chicken)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Structural component of eye lens gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.
  • 基因功能參考文獻：
    1. the role of the charged residues at the end of TM-1 in voltage sensing in Cx26, Cx46, and Cx50. PMID: 27143357
    2. Cx50 is phosphorylated in vivo by PKA at Ser-395, which promotes increased dye permeability of Cx50 channels and plays an important role in metabolic coupling and transport in lens fibers PMID: 21454606
    3. Val(362) is important for maintaining the helical structure and is crucial for the role of Cx50 in promoting lens epithelial-fiber differentiation PMID: 20395299
    4. These data suggest that the N-terminus plays a critical role in determining many of biophysical properties of Cx45.6 gap junctional channels, including molecular permeability and voltage gating. PMID: 16931554
    5. study concludes that the expression of Cx45.6, but not Cx45.6-dependent gap junction channels, is involved in lens epithelial-fiber cell differentiation, and the C-terminal domain of Cx45.6 plays a predominant role in mediating this process PMID: 17895360
    6. Phosphorylation at Ser(364) appears to stimulate turnover primarily through proteasome pathway; this phosphorylation inhibits cleavage by caspase-3. PMID: 18649174
    7. Human Cx50 is functionally involved in cell differentiation and lens development, and the E48K mutant promotes primary lens cell differentiation indistinguishable from wild-type chicken Cx50, despite its lack of junctional channel function PMID: 19126675

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
  • 蛋白家族：
    Connexin family, Alpha-type (group II) subfamily
  • 數(shù)據(jù)庫鏈接：


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