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  4. Recombinant Canine parvovirus type 2 Non-capsid protein NS-1 (NS1), partial

Recombinant Canine parvovirus type 2 Non-capsid protein NS-1 (NS1), partial

  • 中文名稱：
    Recombinant Canine parvovirus type 2 Non-capsid protein NS-1(NS1) ,partial
  • 貨號：
    CSB-YP319470CCS
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Canine parvovirus type 2 Non-capsid protein NS-1(NS1) ,partial
  • 貨號：
    CSB-EP319470CCS
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Canine parvovirus type 2 Non-capsid protein NS-1(NS1) ,partial
  • 貨號：
    CSB-EP319470CCS-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Canine parvovirus type 2 Non-capsid protein NS-1(NS1) ,partial
  • 貨號：
    CSB-BP319470CCS
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Canine parvovirus type 2 Non-capsid protein NS-1(NS1) ,partial
  • 貨號：
    CSB-MP319470CCS
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NS1
  • Uniprot No.：
  • 別名：
    NS1; Initiator protein NS1; NS1; EC 3.1.21.-; EC 3.6.4.12; NCVP1; Non-capsid protein NS-1; Non-structural protein 1; Non-structural protein NS1
  • 種屬：
    Canine parvovirus type 2 (isolate Dog/United States/CPV-N/1978) (CPV-2)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. The helicase activity unwinds DNA in a 3'-5' direction on the longer strand. Inhibits the host cell cycle during the G1/S transition, the S-phase, and the G2/M transition. These arrests may provide essential cellular factors for viral DNA replication. Promotes apoptosis in host cell.
  • 基因功能參考文獻：
    1. Increase in caspase-3 activity and no change in p53 activity with time in Canine Parvovirus-2 NS1 expressing HeLa cells showed the induction of apoptosis to be caspase dependent and p53 independent. PMID: 23416147
    2. NS1 of canine parvovirus induces apoptosis of cells. PMID: 22712408
    3. These data suggest that DNA binding of NS1 is dependent on its ability to both bind and hydrolyze ATP. PMID: 20219935
  • 亞細胞定位：
    Host nucleus.
  • 蛋白家族：
    Parvoviruses non-capsid protein family
  • 數據庫鏈接：

    KEGG: vg:1724588



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