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Recombinant Bovine Serum amyloid A protein (SAA1)

In Stock
  • 中文名稱:
    牛SAA1重組蛋白
  • 貨號:
    CSB-EP020656BO
  • 規格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產品詳情

  • 純度:
    Greater than 85% as determined by SDS-PAGE.
  • 基因名:
  • Uniprot No.:
  • 別名:
    SAA1; SAA; Serum amyloid A protein; SAA) [Cleaved into: Amyloid protein A; Amyloid fibril protein AA)]
  • 種屬:
    Bos taurus (Bovine)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    30.1 kDa
  • 表達區域:
    19-130aa
  • 氨基酸序列
    QWMSFFGEAYEGAKDMWRAYSDMREANYKGADKYFHARGNYDAAQRGPGGAWAAKVISDARENIQRFTDPLFKGTTSGQGQEDSRADQAANEWGRSGKDPNHFRPAGLPDKY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽:
    N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged
  • 產品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    Tris-based buffer,50% glycerol
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產品描述:

    The expression of recombinant Bovine SAA1 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis-SUMO tag and C-terminal Myc tag sequence to the designated sequence encoding the 19-130aa of the Bovine SAA1 protein. This N-terminal 6xHis-tagged recombinant Bovine SAA1 protein is also characterized by high purity, >85%. Under SDS-PAGE condition, this recombinant SAA1 protein migrated to the band of about 33 kDa molecular weight.

    SAA1 protein is expressed primarily in the liver and circulates in low levels in the blood. Although its function is not fully understood, serum amyloid A1 appears to play a role in the immune system. SAA1 is closely associated with high-density lipoprotein. Additionally, SAA1 interacts with a number of mammalian proteins. These receptors include the G protein-coupled chemoattractant receptor FPR2. Certain studies indicated that SAA1 might contribute to the development of atherosclerosis. SAA1 is also presented in macrophages and epithelial cells in various tissues. It has been found to enhance local Th17 response in the gut. Other studies have shown that native human SAA1 retains some of the cytokine-like activities such as the G-CSF-induction capability. In a case, the findings suggested that a mutation in the SAA1 promoter causes hereditary amyloid A amyloidosis. Besides, as SAA1 transcriptionally activated by STAT3, it could accelerate renal interstitial fibrosis by inducing endoplasmic reticulum stress; SAA1knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling.

  • Datasheet & COA:
    Please contact us to get it.

產品評價

問答及客戶評論

 常見問題解答
Q:

CSB-EP020656BO 客戶問為什么MW是33 kDa,很多文獻上寫的是12kDa

A:
CSB-EP020656BO N-terminal 6xHis-SUMO-tagged and C-terminal Myc-tagged, CSB-EP020656BOb3 N-terminal 10xHis-SUMO-tagged and C-terminal Myc-taggedd, CSB-EP020656BOa0 N-terminal 6xHis-tagged, 我們的分子量大小是包含標簽的分子量大小的,您在文獻中看到的12kd,指的應該是19-130aa,無標簽對應的僅目的蛋白的分子量大小,若您想要小標簽的話,優先推薦CSB-EP020656BOa0 N-terminal 6xHis-tagged。

靶點詳情

  • 功能:
    Major acute phase reactant. Apolipoprotein of the HDL complex.
  • 基因功能參考文獻:
    1. polymorphisms in the SAA2 gene are associated with milk production traits in Chinese Holstein cows PMID: 26373797
  • 相關疾?。?/div>
    Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    SAA family
  • 組織特異性:
    Expressed by the liver; secreted in plasma.
  • 數據庫鏈接:


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