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  4. Recombinant Bovine Repressor of RNA polymerase III transcription MAF1 homolog (MAF1)

Recombinant Bovine Repressor of RNA polymerase III transcription MAF1 homolog (MAF1)

  • 中文名稱：
    牛MAF1重組蛋白
  • 貨號：
    CSB-YP013313BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛MAF1重組蛋白
  • 貨號：
    CSB-EP013313BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛MAF1重組蛋白
  • 貨號：
    CSB-EP013313BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛MAF1重組蛋白
  • 貨號：
    CSB-BP013313BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛MAF1重組蛋白
  • 貨號：
    CSB-MP013313BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAF1
  • Uniprot No.：
  • 別名：
    MAF1Repressor of RNA polymerase III transcription MAF1 homolog
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-260
  • 氨基酸序列
    MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGED EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKALKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEDEE EEEESGGGGS EGGPEEPGTM EEDRVPVICM
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance. Plays also a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation. Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP.
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    MAF1 family
  • 數據庫鏈接：


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