Recombinant Bovine Peptidyl-glycine alpha-amidating monooxygenase (PAM), partial
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中文名稱:牛PAM重組蛋白
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貨號(hào):CSB-YP017417BO
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規(guī)格:
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來(lái)源:Yeast
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其他:
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中文名稱:牛PAM重組蛋白
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貨號(hào):CSB-EP017417BO
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規(guī)格:
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來(lái)源:E.coli
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其他:
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中文名稱:牛PAM重組蛋白
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貨號(hào):CSB-EP017417BO-B
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規(guī)格:
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來(lái)源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
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中文名稱:牛PAM重組蛋白
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貨號(hào):CSB-BP017417BO
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規(guī)格:
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來(lái)源:Baculovirus
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其他:
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中文名稱:牛PAM重組蛋白
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貨號(hào):CSB-MP017417BO
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規(guī)格:
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來(lái)源:Mammalian cell
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:PAM
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Uniprot No.:
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別名:PAMPeptidyl-glycine alpha-amidating monooxygenase; PAM) [Includes: Peptidylglycine alpha-hydroxylating monooxygenase; PHM; EC 1.14.17.3); Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; EC 4.3.2.5; Peptidylamidoglycolate lyase; PAL)]
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種屬:Bos taurus (Bovine)
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蛋白長(zhǎng)度:Partial
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點(diǎn)詳情
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功能:Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate.
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基因功能參考文獻(xiàn):
- These data suggest that cortisol affects oxytocin synthesis and secretion in bovine ovarian cells, by acting on the expression of key genes, that may impair ovary PMID: 23971190
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亞細(xì)胞定位:Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein.
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蛋白家族:Peptidyl-alpha-hydroxyglycine alpha-amidating lyase family; Copper type II ascorbate-dependent monooxygenase family
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數(shù)據(jù)庫(kù)鏈接:
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