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  4. Recombinant Bovine N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD)

Recombinant Bovine N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPEPLD)

  • 中文名稱：
    牛NAPEPLD重組蛋白
  • 貨號：
    CSB-YP701505BO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛NAPEPLD重組蛋白
  • 貨號：
    CSB-EP701505BO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛NAPEPLD重組蛋白
  • 貨號：
    CSB-EP701505BO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛NAPEPLD重組蛋白
  • 貨號：
    CSB-BP701505BO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛NAPEPLD重組蛋白
  • 貨號：
    CSB-MP701505BO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NAPEPLD
  • Uniprot No.：
  • 別名：
    NAPEPLD; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D; EC 3.1.4.54
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-392
  • 氨基酸序列
    MDENETNQLL MTSNQYPKEA VRKRQNSRNS GGSDSSRFSR KSFKLDYRLE EDVTKSKKGK DGRFVNPWPT WKNPSIPSLL RWVITERDHS SVPCSKELDK ELPVLKPYFI DDPEEAGVRG AGLRVTWLGH ATVMVEMDEL ILLTDPIFSA RASPSQRMGP KRFRRAPCTV EELPRIDAVL VSHNHYDHLD CNSVIALNER FGNELRWFVP LGLLDWMQKC GCENVIELDW WEENCVPGHD KVTFVFTPSQ HWCKRTLMDD NKVLWGSWSV LGPWNRFFFA GDTGYCSAFE EIGKRFGPFD LAAIPIGAYE PRWFMKYQHV DPEEAVKIHI DVQAKKSVAI HWGTFALANE HYLEPPAKLC EALEKYRLKT EDFLVLKHGE SRYLNTDDED VE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    D-type phospholipase that hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce bioactive N-acylethanolamines/fatty acid ethanolamides (NAEs/FAEs) and phosphatidic acid. Cleaves the terminal phosphodiester bond of diacyl- and alkenylacyl-NAPEs, primarily playing a role in the generation of long-chain saturated and monounsaturated NAEs in the brain. May control NAPE homeostasis in dopaminergic neuron membranes and regulate neuron survival, partly through RAC1 activation. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose. Has limited D-type phospholipase activity toward N-acyl lyso-NAPEs.
  • 亞細胞定位：
    Golgi apparatus membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Nucleus envelope. Nucleus, nucleoplasm.
  • 蛋白家族：
    NAPE-PLD family
  • 組織特異性：
    Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
  • 數據庫鏈接：


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