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  4. Recombinant Bovine Homeobox protein NANOG (NANOG)

Recombinant Bovine Homeobox protein NANOG (NANOG)

  • 中文名稱：
    牛NANOG重組蛋白
  • 貨號：
    CSB-YP678046BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛NANOG重組蛋白
  • 貨號：
    CSB-EP678046BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛NANOG重組蛋白
  • 貨號：
    CSB-EP678046BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛NANOG重組蛋白
  • 貨號：
    CSB-BP678046BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛NANOG重組蛋白
  • 貨號：
    CSB-MP678046BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-300
  • 氨基酸序列
    MSVGPACPQS LLGPEASNSR ESSPMPEESY VSLQTSSADT LDTDTVSPLP SSMDLLIQDS PDSSTSPRVK PLSPSVEEST EKEETVPVKK QKIRTVFSQT QLCVLNDRFQ RQKYLSLQQM QELSNILNLS YKQVKTWFQN QRMKCKKWQK NNWPRNSNGM PQGPAMAEYP GFYSYHQGCL VNSPGNLPMW GNQTWNNPTW SNQSWNSQSW SNHSWNSQAW CPQAWNNQPW NNQFNNYMEE FLQPGIQLQQ NSPVCDLEAT LGTAGENYNV IQQTVKYFNS QQQITDLFPN YPLNIQPEDL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator and repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
  • 基因功能參考文獻：
    1. NANOG was absent or very faint in OCT4 knockout blastocysts. PMID: 29483258
    2. analysis of pluripotency gene expression of OCT4, SOX2 and NANOG and mRNA levels of some of their downstream targets in bovine oocytes and early embryos PMID: 22479535
    3. essential for induction of pluripotency in adult fibroblasts PMID: 21478453
    4. Nanog displayed relatively the same methylation levels between sperm and oocytes PMID: 20604984
    5. Noggin, a cytokine inhibiting the BMP4 pathway, successfully upregulated the relative expression of NANOG mRNA in the ICM explants with respect to controls. PMID: 19594391

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Nanog homeobox family
  • 數據庫鏈接：


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