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  4. Recombinant Bovine Glutamate dehydrogenase 1, mitochondrial (GLUD1), partial

Recombinant Bovine Glutamate dehydrogenase 1, mitochondrial (GLUD1), partial

  • 中文名稱：
    牛GLUD1重組蛋白
  • 貨號：
    CSB-YP009544BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛GLUD1重組蛋白
  • 貨號：
    CSB-EP009544BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛GLUD1重組蛋白
  • 貨號：
    CSB-EP009544BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛GLUD1重組蛋白
  • 貨號：
    CSB-BP009544BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛GLUD1重組蛋白
  • 貨號：
    CSB-MP009544BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    GLUD1; GLUD; Glutamate dehydrogenase 1; mitochondrial; GDH 1; EC 1.4.1.3
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Partial
  • 表達區域：
    58-558
  • 氨基酸序列
    ADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLK TRETEEQKRN RVRSILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGVD VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCITV GESDGSIWNP DGIDPKELED FKLQHGTILG FPKAKIYEGS ILEVDCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLNNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFRVY NEAGVTFT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. Plays a role in insulin homeostasis. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
  • 基因功能參考文獻：
    1. near-atomic resolution cryoelectron microscopy structures, at resolutions ranging from 3.2 A to 3.6 A for GDH complexes, is reported. PMID: 27036132
    2. Data show that glutamate dehydrogenase activity in an enzymatic catalyzed reaction by tracing the increasing of oxidation current of NADH. PMID: 20675102
    3. Data show that heat inactivation of the native GDH enzyme occurred with a rate constant of inactivation of 0.252 min-1. PMID: 20665690
    4. the existence of two partially unfolded states of GDH at moderate acidic pHs which may be considered as molten and pre-molten globule-like states. PMID: 17517789
    5. Results showt superactivation is caused by cleavage at the N terminus of the protein and not the C-terminal allosteric site, as has previously been suggested. PMID: 18467497

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  • 亞細胞定位：
    Mitochondrion. Endoplasmic reticulum.
  • 蛋白家族：
    Glu/Leu/Phe/Val dehydrogenases family
  • 數據庫鏈接：


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