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  4. Recombinant Bovine Endonuclease G, mitochondrial (ENDOG)

Recombinant Bovine Endonuclease G, mitochondrial (ENDOG)

  • 中文名稱：
    牛ENDOG重組蛋白
  • 貨號：
    CSB-YP007665BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛ENDOG重組蛋白
  • 貨號：
    CSB-EP007665BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛ENDOG重組蛋白
  • 貨號：
    CSB-EP007665BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛ENDOG重組蛋白
  • 貨號：
    CSB-BP007665BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛ENDOG重組蛋白
  • 貨號：
    CSB-MP007665BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ENDOGEndonuclease G; mitochondrial; Endo G; EC 3.1.30.-
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    49-299
  • 氨基酸序列
    AG LPAVPGAPAG GGPGELAKYG LPGVAQLKSR ASYVLCYDPR TRGALWVVEQ LRPEGLRGDG NRSSCDFHED DSVHAYHRAT NADYRGSGFD RGHLAAAANH RWSQKAMDDT FYLSNVAPQV PHLNQNAWNN LEKYSRSLTR TYQNVYVCTG PLFLPRTEAD GKSYVKYQVI GKNHVAVPTH FFKVLILEAA GGQIELRSYV MPNAPVDEAI PLEHFLVPIE SIERASGLLF VPNILARAGS LKAITAGSK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA. The 5hmC-modified nucleotide does not increase the binding affinity, but instead increases the efficiency of cutting and specifies the site of cleavage for the modified DNAs. Shows significantly higher affinity for four- stranded Holliday junction over duplex and single-stranded DNAs. Promotes conservative recombination when the DNA is 5hmC-modified. Promotes autophagy through the suppression of mTOR by its phosphorylation-mediated interaction with YWHAG and its endonuclease activity-mediated DNA damage response. GSK3-beta mediated phosphorylation of ENDOG enhances its interaction with YWHAG, leading to the release of TSC2 and PIK3C3 from YWHAG resulting in mTOR pathway suppression and autophagy initiation. Promotes cleavage of mtDNA in response to oxidative and nitrosative stress, in turn inducing compensatory mtDNA replication.
  • 基因功能參考文獻：
    1. Results describe the mechanism of catalysis and substrate binding by the apoptotic mitochondrial nuclease EndoG, which belongs to the large family of DNA/RNA non-specific betabetaalpha-Me-finger nucleases. PMID: 15066427
  • 亞細胞定位：
    Mitochondrion.
  • 蛋白家族：
    DNA/RNA non-specific endonuclease family
  • 數據庫鏈接：


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