在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Bovine E3 ubiquitin-protein ligase RNF8 (RNF8)

  • 中文名稱:
    牛RNF8重組蛋白
  • 貨號:
    CSB-YP644138BO
  • 說明書:
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    牛RNF8重組蛋白
  • 貨號:
    CSB-EP644138BO
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    牛RNF8重組蛋白
  • 貨號:
    CSB-EP644138BO-B
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    牛RNF8重組蛋白
  • 貨號:
    CSB-BP644138BO
  • 說明書:
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    牛RNF8重組蛋白
  • 貨號:
    CSB-MP644138BO
  • 說明書:
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    RNF8
  • Uniprot No.:
  • 別名:
    RNF8; E3 ubiquitin-protein ligase RNF8; EC 2.3.2.27; RING finger protein 8; RING-type E3 ubiquitin transferase RNF8
  • 種屬:
    Bos taurus (Bovine)
  • 蛋白長度:
    full length protein
  • 表達(dá)區(qū)域:
    1-487
  • 氨基酸序列
    MGDPGSLVTE GRAGERSWCL RRVGMNTEWL LLEDGNEVTV GRGFGVTYQL VSKICPLMIS RNHCILKQNA EGQWTIKDNK SLNGVWLNRE RLEPLKVYSI HKGDHIQLGV PLENKENAEY EYEVTEEDWE RIYPCLSPKS DQMMEKNKGL RTKRKFSLDE LEGSGAEGPS NLKSKISKLS CEPGQQVKSH GKGKVASQPS EYLDPKLTSF EPSVKTTGAH VNPGPAKVIE LLRKKKKASN PSASQSSLEL FKVTMSRILM LKTQMQEKQV AVLNVKKQTK KGSSKKIVKM EQELQDLQSQ LCAEQAQQQA RVEQLEKTIQ EEQQHLEGLE KEEGEEDLKQ QLAQALQEYR SLVEELNRSK KNFEAIIQAK DKELEQTKEE KEKVQAQKEE VLSHMNDVLE NELQCIICSE YFVEAVTLNC AHSFCSYCIN EWMKRKVECP ICRKDIKSKT RSLVLDNCIS KMVDNLNSEV KERRIVLIRE RKGKRLF
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能:
    E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites. Following DNA DSBs, it is recruited to the sites of damage by ATM-phosphorylated MDC1 and catalyzes the 'Lys-63'-linked ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Also controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites. Also recruited at DNA interstrand cross-links (ICLs) sites and catalyzes 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Promotes the formation of 'Lys-63'-linked polyubiquitin chains via interactions with the specific ubiquitin-conjugating UBE2N/UBC13 and ubiquitinates non-histone substrates such as PCNA. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Also catalyzes the formation of 'Lys-48'-linked polyubiquitin chains via interaction with the ubiquitin-conjugating UBE2L6/UBCH8, leading to degradation of substrate proteins such as CHEK2, JMJD2A/KDM4A and KU80/XRCC5: it is still unclear how the preference toward 'Lys-48'- versus 'Lys-63'-linked ubiquitination is regulated but it could be due to RNF8 ability to interact with specific E2 specific ligases. For instance, interaction with phosphorylated HERC2 promotes the association between RNF8 and UBE2N/UBC13 and favors the specific formation of 'Lys-63'-linked ubiquitin chains. Promotes non-homologous end joining (NHEJ) by promoting the 'Lys-48'-linked ubiquitination and degradation the of KU80/XRCC5. Following DNA damage, mediates the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF168, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Following DNA damage, mediates the ubiquitination and degradation of POLD4/p12, a subunit of DNA polymerase delta. In the absence of POLD4, DNA polymerase delta complex exhibits higher proofreading activity. In addition to its function in damage signaling, also plays a role in higher-order chromatin structure by mediating extensive chromatin decondensation. Involved in the activation of ATM by promoting histone H2B ubiquitination, which indirectly triggers histone H4 'Lys-16' acetylation (H4K16ac), establishing a chromatin environment that promotes efficient activation of ATM kinase. Required in the testis, where it plays a role in the replacement of histones during spermatogenesis. At uncapped telomeres, promotes the joining of deprotected chromosome ends by inducing H2A ubiquitination and TP53BP1 recruitment, suggesting that it may enhance cancer development by aggravating telomere-induced genome instability in case of telomeric crisis. Promotes the assembly of RAD51 at DNA DSBs in the absence of BRCA1 and TP53BP1 Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
  • 亞細(xì)胞定位:
    Nucleus. Cytoplasm. Midbody. Chromosome, telomere.
  • 蛋白家族:
    RNF8 family
  • 數(shù)據(jù)庫鏈接:


主站蜘蛛池模板: 亚洲乱码精品久久久久..| 亚洲国产成人久久一区www| 日本高清www午色夜在线视频| 韩国精品久久久久久无码| 中文字幕高清免费日韩视频在线| 国产漂亮白嫩美女在线观看| 丰满亚洲大尺度无码无码专线| 在线观看免费网页欧美成| 精品无码专区毛片| 国产又黄又爽又色的免费| 精品无码国产污污污免费| 亚洲 欧美 日韩 综合aⅴ电影| 少妇中文字幕乱码亚洲影视| 怀孕挺大肚子疯狂高潮av毛片| 色一情一区二区三区四区| 国产精成人品日日拍夜夜| 久久久久噜噜噜亚洲熟女综合| 麻豆果冻传媒2021精品传媒一区下载 | 久久精品国产精品亚洲色婷婷| 久久婷婷丁香七月色综合| 国产成年无码v片在线| 国产婷婷亚洲999精品小说| 一区二区三区视频| 蜜桃mv在线播放免费观看视频| 人妻无码中文字幕| 亚洲精品久久久www小说| 日本熟妇色一本在线观看| 中文精品无码中文字幕无码专区| 蜜臀av性久久久久蜜臀aⅴ| 色婷婷狠狠97成为人免费| 18禁裸乳无遮挡啪啪无码免费| 欧洲女人牲交视频免费| 国产乱人伦偷精品视频免| 伊人久久精品在热线热| 亚洲人成电影网站色www| 午夜男女刺激爽爽影院| 美女视频黄的全免费视频网站| 日本电影一区二区三区| 成在人线无码aⅴ免费视频| 中文字幕日韩精品亚洲一区| 国产免费永久精品无码|