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  4. Recombinant Bovine Cytochrome b5 (CYB5A), partial

Recombinant Bovine Cytochrome b5 (CYB5A), partial

  • 中文名稱：
    Recombinant Bovine Cytochrome b5(CYB5A) ,partial
  • 貨號：
    CSB-YP006309BO1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bovine Cytochrome b5(CYB5A) ,partial
  • 貨號：
    CSB-EP006309BO1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bovine Cytochrome b5(CYB5A) ,partial
  • 貨號：
    CSB-EP006309BO1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bovine Cytochrome b5(CYB5A) ,partial
  • 貨號：
    CSB-BP006309BO1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bovine Cytochrome b5(CYB5A) ,partial
  • 貨號：
    CSB-MP006309BO1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CYB5A
  • Uniprot No.：
  • 別名：
    CYB5A; CYB5; Cytochrome b5
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.
  • 基因功能參考文獻：
    1. the conserved Tyr30 in Cyt b5 is not replaceable due to the presence of both the H-bond network and hydrophobic interaction in the secondary sphere of the heme active site. PMID: 27888781
    2. The study computes electron transfer rates for the [myoglobin(wt), cytochrome b5] complex. PMID: 22955681
    3. The solution structure of the oxidized cytochrome b(5) mutant V61H. PMID: 12893266
    4. the effects on the heme microenvironment of cytochrome b(5) caused by the mutation from Val45 to Tyr45, His45 and Glu45 PMID: 14644556
    5. Results from three combined experiments demonstrate that the conserved negatively charged region of cytb5 surrounding the solvent-exposed heme edge is involved in the binding interaction with horse heart cytochrome c. PMID: 14674751
    6. From a series of site-directed mutations on Cyt b5 this report assesses the contributions of amino acid residues that are expected to influence the docking and electron transfer (ET) between Cyt b5 and horse heart Cyt c. PMID: 15035623
    7. simulations provided qualitative microscopic explanations of many of the differences in physical properties between outer mitochondrial membrane CYB5 isoform and microsomal CYB5 and two mutants in terms of localized changes in structure and flexibility PMID: 16807901

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Cytochrome b5 family
  • 數據庫鏈接：


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