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  4. Recombinant Bovine Argininosuccinate synthase (ASS1)

Recombinant Bovine Argininosuccinate synthase (ASS1)

  • 中文名稱：
    Recombinant Bovine Argininosuccinate synthase(ASS1)
  • 貨號：
    CSB-YP002234BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bovine Argininosuccinate synthase(ASS1)
  • 貨號：
    CSB-EP002234BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bovine Argininosuccinate synthase(ASS1)
  • 貨號：
    CSB-EP002234BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bovine Argininosuccinate synthase(ASS1)
  • 貨號：
    CSB-BP002234BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bovine Argininosuccinate synthase(ASS1)
  • 貨號：
    CSB-MP002234BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ASS1
  • Uniprot No.：
  • 別名：
    ASS1Argininosuccinate synthase; EC 6.3.4.5; Citrulline--aspartate ligase
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full length protein
  • 表達區域：
    1-412
  • 氨基酸序列
    MSGKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF IEDISKEFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG KGNDQIRFEL TCYSLAPQIK VIAPWRMPEF YNRFQGRNDL MEYAKQHGIP VPVTPKNPWS MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNSP DMLEIEFKKG VPVKVTNVGD GTTHSTALEL FLYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPVDATG FININSLRLK EYHRLQNKVT AK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
  • 基因功能參考文獻：
    1. calcium-dependent phosphorylation of argininosuccinate synthase Ser-328 is mediated by PKCalpha PMID: 22696221
    2. dose-dependent reduction of ASS as a result of siRNA treatment corresponded to diminished capacity to produce NO, despite saturating levels of arginine in the medium; reduced expression of ASS resulted in loss of viability due to apoptosis PMID: 14970240
    3. the function of the extended 5'-UTRs of AS mRNA was investigated PMID: 15851478
    4. In endothelial cells TNF-alpha coordinately downregulates nitric oxide synthase (eNOS) and argininosuccinate synthase expression, resulting in a severely impaired citrulline-NO cycle. PMID: 17496212
    5. These results represent the first demonstration that vascular endothelial nitric oxide production can be regulated by dynamic argininosuccinate synthase phosphorylation. PMID: 18948083

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  • 相關疾病：
    Defects in ASS1 are the cause of a bovine form of citrullinemia.
  • 亞細胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Argininosuccinate synthase family, Type 1 subfamily
  • 數據庫鏈接：


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