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  4. Recombinant Bacillus subtilis Stage 0 sporulation protein A (spo0A)

Recombinant Bacillus subtilis Stage 0 sporulation protein A (spo0A)

  • 中文名稱：
    枯草芽孢桿菌spo0A重組蛋白
  • 貨號：
    CSB-YP361864BRJ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0A重組蛋白
  • 貨號：
    CSB-EP361864BRJ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0A重組蛋白
  • 貨號：
    CSB-EP361864BRJ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0A重組蛋白
  • 貨號：
    CSB-BP361864BRJ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌spo0A重組蛋白
  • 貨號：
    CSB-MP361864BRJ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    spo0A
  • Uniprot No.：
  • 別名：
    spo0A; spo0C; spo0G; BSU24220; Stage 0 sporulation protein A; Stage 0 sporulation protein C; Stage 0 sporulation protein G
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-267
  • 氨基酸序列
    MEKIKVCVAD DNRELVSLLS EYIEGQEDME VIGVAYNGQE CLSLFKEKDP DVLVLDIIMP HLDGLAVLER LRESDLKKQP NVIMLTAFGQ EDVTKKAVDL GASYFILKPF DMENLVGHIR QVSGNASSVT HRAPSSQSSI IRSSQPEPKK KNLDASITSI IHEIGVPAHI KGYLYLREAI SMVYNDIELL GSITKVLYPD IAKKFNTTAS RVERAIRHAI EVAWSRGNID SISSLFGYTV SMTKAKPTNS EFIAMVADKL RLEHKAS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the expression of some genes that are critical to the sporulation process. Repressor of abrB, activator of the spoIIa operon. Binds the DNA sequence 5'-TGNCGAA-3' (0A box).
  • 基因功能參考文獻：
    1. Cells exhibit a constant probability of attaining a high threshold level of Spo0A~P due to fluctuations in the flux of phosphoryl groups through the phosphorelay. PMID: 28838935
    2. The pulsed behavior of Spo0A~P allows the introduction of a time delay between the activations of KinA and Spo0A~P. (Review) PMID: 26941227
    3. Spo0A regulates chromosome copy number during sporulation by binding to the origin of replication. PMID: 23301687
    4. Spo0A synthes is governed by a promoter switching mechanism during different growth stages. PMID: 21949067
    5. The increase in spo0A transcription during early growth phase results in the synthesis of phosphorylated Spo0A. PMID: 21552330
    6. responsible for generating a bistable response resulting in phenotypic variation within the sporulating culture PMID: 15916600
    7. aspartate 43 has a critical role in Spo0E catalytic activity, whereas the other residues have a role in protein conformation and/or interaction with Spo0A PMID: 18045868
    8. kinC and kinD are responsible for expression of spo0A during logarithmical growth that are not sufficient to activate sporulation, but sufficient for suppression of the lytic cycle of phi 29. PMID: 18410285

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 數(shù)據(jù)庫鏈接：


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