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  4. Recombinant Bacillus subtilis HPr kinase/phosphorylase (hprK)

Recombinant Bacillus subtilis HPr kinase/phosphorylase (hprK)

  • 中文名稱：
    枯草芽孢桿菌hprK重組蛋白
  • 貨號：
    CSB-YP518483BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌hprK重組蛋白
  • 貨號：
    CSB-EP518483BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌hprK重組蛋白
  • 貨號：
    CSB-EP518483BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌hprK重組蛋白
  • 貨號：
    CSB-BP518483BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌hprK重組蛋白
  • 貨號：
    CSB-MP518483BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    hprK
  • Uniprot No.：
  • 別名：
    hprK; ptsK; yvoB; BSU35000; HPr kinase/phosphorylase; HPrK/P; EC 2.7.11.-; EC 2.7.4.-; HPr kinase/phosphatase; HPr(Ser) kinase/phosphorylase
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-310
  • 氨基酸序列
    AKVRTKDVM EQFNLELISG EEGINRPITM SDLSRPGIEI AGYFTYYPRE RVQLLGKTEL SFFEQLPEEE KKQRMDSLCT DVTPAIILSR DMPIPQELID ASEKNGVPVL RSPLKTTRLS SRLTNFLESR LAPTTAIHGV LVDIYGVGVL ITGKSGVGKS ETALELVKRG HRLVADDCVE IRQEDQDTLV GNAPELIEHL LEIRGLGIIN VMTLFGAGAV RSNKRITIVM NLELWEQGKQ YDRLGLEEET MKIIDTEITK LTIPVRPGRN LAVIIEVAAM NFRLKRMGLN AAEQFTNKLA DVIEDGEQEE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of 'Ser-45' in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Also phosphorylates/dephosphorylates the HPr-like catabolite repression protein crh on 'Ser-46'. Therefore, by controlling the phosphorylation state of HPr and crh, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion.
  • 基因功能參考文獻：
    1. results suggest that very small amounts of HPrK/P are sufficient for complete carbon catabolite repression and that control of HPrK/P activity depends only on the presence of effectors and not on the abundance of the enzyme PMID: 16684115
  • 蛋白家族：
    HPrK/P family
  • 數據庫鏈接：


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