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  4. Recombinant Bacillus subtilis Gamma-DL-glutamyl hydrolase (pgdS)

Recombinant Bacillus subtilis Gamma-DL-glutamyl hydrolase (pgdS)

  • 中文名稱：
    枯草芽孢桿菌pgdS重組蛋白
  • 貨號：
    CSB-YP309870BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌pgdS重組蛋白
  • 貨號：
    CSB-EP309870BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌pgdS重組蛋白
  • 貨號：
    CSB-EP309870BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌pgdS重組蛋白
  • 貨號：
    CSB-BP309870BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌pgdS重組蛋白
  • 貨號：
    CSB-MP309870BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    pgdS
  • Uniprot No.：
  • 別名：
    pgdS; ywtD; BSU35860; Gamma-DL-glutamyl hydrolase; EC 3.4.19.-; Poly-gamma-glutamate depolymerase; PGA depolymerase
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    33-413
  • 氨基酸序列
    DTSSELIV SEAKNLLGYQ YKYGGETPKE GFDPSGLIQY VFSKADIHLP RSVNDQYKIG TAVKPENLKP GDILFFKKEG STGTVPTHDA LYIGDGQMVH STQSKGVIIT NYKKSSYWSG TYIGARRIAA DPATADVPVV QEAEKYIGVP YVFGGSTPSE GFDCSGLVQY VFQQALGIYL PRSAEQQWAV GEKVAPQNIK PGDVVYFSNT YKTGISHAGI YAGAGRFIQA SRSEKVTISY LSEDYWKSKM TGIRRFDNLT IPKENPIVSE ATLYVGEVPY KQGGVTPETG FDTAGFVQYV YQKAAGISLP RYATSQYNAG TKIEKADLKP GDIVFFQSTS LNPSIYIGNG QVVHVTLSNG VTITNMNTST YWKDKYAGSI RVQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA).
  • 基因功能參考文獻：
    1. Low-resolution architecture of PgdS hydrolase from Bacillus subtilis in solution using small angle X-ray scattering (SAXS) method. Combining with other methods, like dynamic light scattering and mutagenesis analyses, a model for the full length structure and the possible substrate delivery route of PgdS are proposed. PMID: 29608608
    2. The knockout of genes for the major poly(gamma-glutamic acid) degrading enzymes, pgdS and ggt, leads to a considerable improvement in polymer yield. PMID: 23335395
  • 亞細胞定位：
    Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133.
  • 蛋白家族：
    Peptidase C40 family
  • 數據庫鏈接：


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