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  4. Recombinant Arabidopsis thaliana Small RNA 2'-O-methyltransferase (HEN1), partial

Recombinant Arabidopsis thaliana Small RNA 2'-O-methyltransferase (HEN1), partial

  • 中文名稱：
    擬南芥HEN1重組蛋白
  • 貨號：
    CSB-YP883685DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥HEN1重組蛋白
  • 貨號：
    CSB-EP883685DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥HEN1重組蛋白
  • 貨號：
    CSB-EP883685DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥HEN1重組蛋白
  • 貨號：
    CSB-BP883685DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥HEN1重組蛋白
  • 貨號：
    CSB-MP883685DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HEN1
  • Uniprot No.：
  • 別名：
    HEN1; CRM2; At4g20910; T13K14.70; Small RNA 2'-O-methyltransferase; EC 2.1.1.n8; Protein CORYMBOSA 2; Protein HUA ENHANCER 1; S-adenosylmethionine-dependent RNA methyltransferase HEN1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Methyltransferase that adds a methyl group to the ribose of the last nucleotide of small RNAs (sRNAs). This protects the 3'-end of sRNAs from uridylation activity and subsequent degradation. Can methylate 3'-end of microRNAs (miRNAs), small interfering RNAs (siRNas) and trans-acting small interfering RNAs (ta-siRNAs). Involved in plant development through its role in small RNAs processing. Required for the specification of reproductive organ identities and the probable repression of A class genes. May control floral determinacy possibly by regulating the expression of the C class floral homeotic gene AGAMOUS (AG).
  • 基因功能參考文獻：
    1. we show HESO1 may also act redundantly with URT1 in miRNA uridylation when HEN1 is fully competent PMID: 25928341
    2. Light-activated HEN1 expression depended on the photoreceptors phytochrome A (phyA), phyB, cryptochrome 1 (cry1), and cry2 and key transcriptional regulators ELONGATED HYPOCOTYL5 (HY5) and HY5-HOMOLOG. PMID: 25052717
    3. Data suggest a mechanism of plant miRNA maturation which involves binding of the HEN1 methyltransferase to the DICER-LIKE 1 ribonuclease DCL1*RNA-binding protein HYL1*miRNA complex. PMID: 25680966
    4. Data indicate that the ago1 mutation in hen1 suppressed micriRNA 3' modifications. PMID: 23839787
    5. AGO1, HYL1, and HEN1 function in proximal-distal and vascular patterning of leaves. PMID: 22623415
    6. Motif mapping analysis revealed that the amino acids located between positions 139 and 320 of Zucchini yellow mosaic virus HC-Pro were associated with Arabidopsis HEN1 interaction. PMID: 21593273
    7. A mutation in RNA-dependent RNA polymerase 2 rescued the defects in miRNA methylation of hen1-2, revealing a previously unsuspected, negative influence of siRNAs on HEN1-mediated miRNA methylation. PMID: 20448024
    8. Kinetic parameters with natural RNA substrates show that HEN1 is highly catalytically efficient in the absence of supplementary proteins. The enzyme modifies individual strands in succession leading to complete methylation of an RNA duplex. PMID: 20705645
    9. HEN1 is a miRNA methyltransferase that adds a methyl group to the 3'-most nucleotide of miRNAs as well as siRNAs from sense transgenes, hairpin transgenes, and transposons or repeat sequences, and a new class of siRNAs known as trans-acting siRNAs. PMID: 16111943
    10. 3.1 A crystal structure of full-length HEN1 from Arabidopsis in complex with a 22-nucleotide small RNA duplex and cofactor product S-adenosyl-l-homocysteine PMID: 19812675

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    Methyltransferase superfamily, HEN1 family
  • 組織特異性：
    Expressed in stems, leaves and inflorescences.
  • 數據庫鏈接：

    KEGG: ath:AT4G20910

    STRING: 3702.AT4G20910.1

    UniGene: At.3574



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