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  4. Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1), partial

Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1 (CPL1), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1(CPL1) ,partial
  • 貨號：
    CSB-YP726407DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1(CPL1) ,partial
  • 貨號：
    CSB-EP726407DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1(CPL1) ,partial
  • 貨號：
    CSB-EP726407DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1(CPL1) ,partial
  • 貨號：
    CSB-BP726407DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana RNA polymerase II C-terminal domain phosphatase-like 1(CPL1) ,partial
  • 貨號：
    CSB-MP726407DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CPL1
  • Uniprot No.：
  • 別名：
    CPL1; FRY2; JOE1; SHI4; At4g21670; F17L22.130; RNA polymerase II C-terminal domain phosphatase-like 1; FCP-like 1; EC 3.1.3.16; Carboxyl-terminal phosphatase-like 1; AtCPL1; CTD phosphatase-like 1; Protein FIERY 2; Protein JASMONATE OVEREXPRESSING 1; Protein SHINY 4
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Processively dephosphorylates 'Ser-5' but not 'Ser-2' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1). This promotes the activity of RNA polymerase II. Together with CPL2, required for male gametes fertility. Multifunctional regulator that modulates plant growth, stress, and phytohormones responses. Negative regulator of stress gene transcription involved in abscisic acid (ABA) mediated and jasmonic acid (JA) mediated signaling pathways, NaCl, osmotic stress, wounding, and cold resistance. Regulates negatively the expression of jasmonic acid (JA) biosynthetic genes in response to wounding. Forms a complex with RCF3 that modulates co-transcriptional processes such as mRNA capping and polyadenylation, and functions to repress stress-inducible gene expression. Dephosphorylates RCF3. Involved in the dephosphorylation of EIF4A3. This dephosphorylation retains EIF4A3 in the nucleus and limits its accumulation in the cytoplasm. Is essential for the degradation of the nonsense-mediated mRNA decay (NMD) transcripts.
  • 基因功能參考文獻：
    1. These results indicate that the coding region of Luciferase is responsible for the cpl1-specific Luciferase overexpression uncoupled with the expression of the endogenous counterpart. PMID: 28692335
    2. A subset of alternatively spliced transcripts and 5'-extended mRNAs with Nonsense-mediated decay (NMD)-eliciting features accumulated in the fry2-1 mutant, cycloheximide-treated wild type, and upf3 mutant plants, indicating that FRY2 is essential for the degradation of these NMD transcripts. PMID: 26887918
    3. RCF2 interacts with the NAC transcription factor NAC019 and that RCF2 dephosphorylates NAC019 PMID: 24415771
    4. These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway. PMID: 24058624
    5. The root-to-shoot translocation of cadmium (Cd) in cpl1-2 is mediated by yet uncharacterized Cd transport mechanisms. PMID: 23455022
    6. FRY2/CPL1 may also recruit specific factors to regulate the co-transcriptional processing of certain transcripts to deal with environmental challenges. PMID: 24146632
    7. we report the identification of a K-homology domain-containing protein named SHINY1 (SHI1) that interacts with CPL1 to modulate gene expression. PMID: 23874224
    8. CPL1 is involved in iron stress responses. PMID: 23144187
    9. In the miRNA processing complex, Serrate functions as a scaffold to mediate CPL1 interaction with HYL1, which needs to be dephosphorylated for optimal activity. In PMID: 23141542
    10. The mechanisms of reading the CTD code by two classes of protein phosphatases, plant CPLs and yeast Ssu72, that specifically dephosphorylate Ser(5) in vitro are reported. PMID: 16148005

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  • 亞細胞定位：
    Nucleus. Nucleus speckle.
  • 組織特異性：
    Expressed at very low levels in roots, leaves, stems, flowers and siliques.
  • 數據庫鏈接：

    KEGG: ath:AT4G21670

    STRING: 3702.AT4G21670.1

    UniGene: At.2700



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