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  4. Recombinant Arabidopsis thaliana RNA-dependent RNA polymerase 6 (RDR6), partial

Recombinant Arabidopsis thaliana RNA-dependent RNA polymerase 6 (RDR6), partial

  • 中文名稱：
    擬南芥RDR6重組蛋白
  • 貨號：
    CSB-YP890330DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RDR6重組蛋白
  • 貨號：
    CSB-EP890330DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RDR6重組蛋白
  • 貨號：
    CSB-EP890330DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RDR6重組蛋白
  • 貨號：
    CSB-BP890330DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RDR6重組蛋白
  • 貨號：
    CSB-MP890330DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RDR6
  • Uniprot No.：
  • 別名：
    RDR6; RDRP6; SDE1; SGS2; At3g49500; T1G12.20; T9C5.95; RNA-dependent RNA polymerase 6; AtRDRP6; EC 2.7.7.48; Protein SILENCING DEFECTIVE 1; Protein SUPPRESSOR OF GENE SILENCING 2; RNA-directed RNA polymerase 6
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    RNA-dependent RNA polymerase involved in post-transcriptional gene silencing (PTGS). Possesses ssRNA and ssDNA-dependent polymerase activity, but does not have priming activity. Possesses in vitro 3' nucleotidyltransferase activity in the presence of UTP as single nucleotide. Required for the production of 21 nucleotide trans-acting small interfering RNAs (ta-siRNAs) derived from TAS1, TAS2 and TAS3 endogenous transcripts. Acts in the RDR6/SGS3/DCL4/AGO7 ta-siRNA pathway involved in leaf developmental timing. Required for the production of natural siRNAs (nat-siRNAs) derived from cis-natural antisense transcripts. Required for the production of 24 nucleotide nat-siRNAs derived from the stress-related P5CDH-SRO5 antisense gene pair. Required for PTGS induced by transgene direct repeats. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL2 and DCL4, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. Involved in the biogenesis of secondary siRNAs which require 22 nucleotide miRNAs associated to AGO1. Participates synergistically with AS1 and AS2 to proper plant development by repressing the miR165 and miR166 microRNAs (independently of AGO10) that may lead to mRNA degradation of genes in the class III HD-ZIP family. Required for the production of some small RNAs derived from the crucifer-infecting tobamovirus (TMV-cg). Required for sense virus-induced post-transcriptional gene silencing (S-PTGS).
  • 基因功能參考文獻(xiàn)：
    1. The results show that the expression of VPg alone, independent of viral infection, is sufficient to induce the degradation of SGS3 and its intimate partner, RDR6. PMID: 27795417
    2. Mungbean yellow mosaic Indian virus AC2 interferes with the biogenesis of siRNA by interacting and blocking the RDR6 activity. PMID: 26433748
    3. Data show that the lethality of decapping mutants is suppressed by impairing RNA-dependent RNA polymerase 6 (RDR6) activity. PMID: 25694514
    4. The Arabidopsis miR472-RDR6 silencing pathway modulates PAMP- and effector-triggered immunity through the post-transcriptional control of disease resistance genes. PMID: 24453975
    5. RDR6-RdDM functions to recognize active Polymerase II-derived Transposable element (TE) mRNA transcripts to both trigger and correctively reestablish TE methylation and epigenetic silencing. PMID: 23542151
    6. RDR6 is required for efficient hpRNA-induced RNA silencing in plants. PMID: 23456296
    7. Genetic interactions during leaf development were observed in a similar way between miR396 overexpression and mutants in RDR6 PMID: 22889666
    8. Compared with intronless transcripts, the spliced transcripts were less effective substrates for RNA-dependent RNA polymerase 6-mediated gene silencing. PMID: 21689169
    9. These data suggest that AGO1 functions differentially with 21- and 22-nt miRNAs to engage the RDR6-associated amplification apparatus. PMID: 20562854
    10. A recessive mutation in RDR6 causes enhanced self-incompatibility. PMID: 19767457
    11. Arabidopsis RNA-Dependent RNA Polymerase (RDR6) gene regulates leaf development. [RDR6] PMID: 16006579
    12. A hypomorphic rdr6 mutant, which does not exhibit the leaf developmental defects observed in null mutants, accumulates wild-type TAS3 ta-siRNA levels but not TAS1 and TAS2 ta-siRNAs, suggesting that TAS3 is required for proper leaf development. PMID: 16682354
    13. Improperly terminated, unpolyadenylated mRNA from transgene transcription is subject to RDR6-mediated RNA silencing, probably by acting as templates for the RNA polymerase, in Arabidopsis thaliana. PMID: 17384170
    14. Several gene transcripts were found to be routed through the RDR6/DCL4-dependent pathway after initial targeting by one or multiple microRNAs or trans-acting short interfering RNA. PMID: 17400893
    15. Biochemical analysis of Arabidopsis RNA-dependent RNA polymerase 6 PMID: 18063577
    16. AGO1 and AGO7 function to ensure efficient clearance of viral RNAs and establish that AGO1 is capable of targeting viral RNAs with more compact structures, whereas AGO7 and RDR6 favor less structured RNA targets PMID: 18799732

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  • 亞細(xì)胞定位：
    Cytoplasmic granule. Nucleus.
  • 蛋白家族：
    RdRP family
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT3G49500

    STRING: 3702.AT3G49500.1

    UniGene: At.65263



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