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  4. Recombinant Arabidopsis thaliana Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 2 (At5g22750), partial

Recombinant Arabidopsis thaliana Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 2 (At5g22750), partial

  • 中文名稱：
    擬南芥RAD5A重組蛋白
  • 貨號：
    CSB-YP875517DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RAD5A重組蛋白
  • 貨號：
    CSB-EP875517DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RAD5A重組蛋白
  • 貨號：
    CSB-EP875517DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RAD5A重組蛋白
  • 貨號：
    CSB-BP875517DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RAD5A重組蛋白
  • 貨號：
    CSB-MP875517DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD5A
  • Uniprot No.：
  • 別名：
    RAD5A; RAD5; At5g22750; MDJ22.17; DNA repair protein RAD5A; EC 3.6.4.-; Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like 2; SMARCA3-like protein 2; RAD5 homolog A; AtRAD5a
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Functions in error-free postreplication DNA repair or DNA-damage tolerance (DTT) pathway. Required for homologous recombination (HR) induced by DNA double-strand break (DSB) in somatic cells. Required for damage-induced DNA repair, independently of MUS81 and RECQL4A. Plays a role in synthesis-dependent strand annealing (SDSA) but not in single-strand annealing (SSA). Possesses double-stranded DNA-dependent ATPase activity. Is able to regress replication forks with preference for forks with a leading strand gap. Is able to catalyze branch migration of Holliday junctions and is unaffected by protein blockades.
  • 基因功能參考文獻：
    1. The helicase domain as well as the RING domain are essential for the function of RAD5A in DNA repair. Further de fi ning the role of RAD5A in the complex network of DNA repair factors, we present evidence that RAD5A acts independently of various other well-known DNA repair pathways. PMID: 28509359
    2. suggesting that AtRAD5a and AtREV3 are required for error-free and translesion synthesis branches of DNA-damage tolerance, respectively. [RAD5a] PMID: 21549648
    3. Ubiquitin-conjugating enzymes and RAD5a proteins are involved in the ubiquitination of plant PCNA at lysine 164. PMID: 23314815
    4. RAD5A and RECQ4A act through two independent pathways for intrastrand cross-link DNA repair. PMID: 20971895
    5. RAD5-dependent error-free branch of postreplication repair is conserved between yeast and plants, and that AtRad5a might be functionally homologous to ScRad5 PMID: 18310306

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    SNF2/RAD54 helicase family, RAD16 subfamily
  • 數據庫鏈接：

    KEGG: ath:AT5G22750

    STRING: 3702.AT5G22750.1

    UniGene: At.31042



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