Recombinant Arabidopsis thaliana Profilin-2 (PRO2)
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中文名稱:擬南芥PRO2重組蛋白
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貨號:CSB-EP669239DOA-B
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說明書:
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規格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產品詳情
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純度:>85% (SDS-PAGE)
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基因名:PRO2
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Uniprot No.:
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別名:PRF2; PFN2; PRO2; At4g29350; F17A13.170; Profilin-2; AtPROF2; AthPRF2
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種屬:Arabidopsis thaliana (Mouse-ear cress)
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蛋白長度:Full Length of Mature Protein
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表達區域:2-131
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氨基酸序列SWQSYVDDH LMCEVEGNHL THAAIFGQDG SVWAQSSAFP QLKPAEIAGI NKDFEEAGHL APTGLFLGGE KYMVVQGEAG AVIRGKKGPG GVTIKKTTQA LVFGIYDEPM TGGQCNLVVE RLGDYLIESG L
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點詳情
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功能:Binds to actin monomers and regulates the organization of the actin cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. At low concentrations, associates with the poly-proline motif of formins to enhance actin filament elongation rate. Binds G-actin and poly-L-proline with low affinity in vitro. Binds ACT1, ACT7 and ACT11 and inhibits actin polymerization. May be involved in the cross-talk between vesicular trafficking and the actin cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. Inhibits cell growth of various pathogenic fungal strains. May play a role as antifungal proteins in the defense system against fungal pathogen attacks.
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基因功能參考文獻:
- Upregulation of profilin 2 by BFA was verified by immunoblot and live imaging at subcellular level. PMID: 21090759
- localized to polygonal meshes resembling the endoplasmic reticulum PMID: 19200149
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亞細胞定位:Cytoplasm, cytoskeleton. Endoplasmic reticulum. Cytoplasm, cytosol. Nucleus.
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蛋白家族:Profilin family
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組織特異性:Expressed in vascular bundles of roots, hypocotyls, cotyledons, leaves, sepals, petals, stamen filaments and stalks of developing seeds. Expressed in leaf epidermal cells, trichomes and stem epidermal cells. Detected in phloem exudates (at protein level).
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