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  4. Recombinant Arabidopsis thaliana Probable WRKY transcription factor 33 (WRKY33), partial

Recombinant Arabidopsis thaliana Probable WRKY transcription factor 33 (WRKY33), partial

  • 中文名稱：
    擬南芥WRKY33重組蛋白
  • 貨號(hào)：
    CSB-YP844600DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥WRKY33重組蛋白
  • 貨號(hào)：
    CSB-EP844600DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥WRKY33重組蛋白
  • 貨號(hào)：
    CSB-EP844600DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥WRKY33重組蛋白
  • 貨號(hào)：
    CSB-BP844600DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥WRKY33重組蛋白
  • 貨號(hào)：
    CSB-MP844600DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    WRKY33
  • Uniprot No.：
  • 別名：
    WRKY33; At2g38470; T19C21.4; Probable WRKY transcription factor 33; WRKY DNA-binding protein 33
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcription factor. Interacts specifically with the W box (5'-TTGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Involved in defense responses. Required for resistance to the necrotrophic fungal pathogen B.cinerea. Regulates the antagonistic relationship between defense pathways mediating responses to the bacterial pathogen P. syringae and the necrotrophic pathogen B.cinerea. Required for the phytoalexin camalexin synthesis following infection with B.cinerea. Acts as positive regulator of the camalexin biosynthetic genes PAD3 (CYP71B15) and CYP71A13 by binding to their promoters. Acts downstream of MPK3 and MPK6 in reprogramming the expression of camalexin biosynthetic genes, which drives the metabolic flow to camalexin production. Functions with WRKY25 as positive regulator of salt stress response and abscisic acid (ABA) signaling. Functions with WRKY25 and WRKY26 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway. The DNA-binding activity of WRKY33 is increased by SIB1 and SIB2.
  • 基因功能參考文獻(xiàn)：
    1. Binding of WRKY18, WRKY40, and WRKY33 to promoters of genes Implicated in microbe-associated molecular patterns (MAMPs)-triggered Immunity. PMID: 28011690
    2. AtWRKY33 has an extended C-terminal domain (CTD) absent in its close homologue AtWRKY25. Both its CTD and the strong pathogen/stress-responsive expression of AtWRKY33 are necessary to complement the critical phenotypes of atwrky33. PMID: 25969555
    3. Loss-of-WRKY33 function resulted in elevated abscisic acid levels and genetic studies confirmed that WRKY33 acts upstream of NCED3/NCED5 to negatively regulate abscisic acid biosynthesis. PMID: 26076231
    4. The WRKY33 proteins interact with VQ motif of SIB1 (SIGMA FACTOR-INTERACTING PROTEIN1) proteins through their C-terminal WRKY domains. PMID: 22535423
    5. direct binding of WRKY33 to the W-boxes in the promoters of ACS2 and ACS6 genes in vivo, suggesting that WRKY33 is directly involved in the activation of ACS2 and ACS6 expression downstream of MPK3/MPK6 cascade in response to pathogen invasion PMID: 22761583
    6. WRKY33 is a negative regulator of the salicylic acid pathway upon Botrytis cinerea infection. PMID: 22392279
    7. Dual-targeted SIB1 and SIB2 function as activators of WRKY33 in plant defense against necrotrophic pathogens. PMID: 21990940
    8. WRKY33 is required for MPK3/MPK6-induced camalexin biosynthesis. PMID: 21498677
    9. MKS1 function and subcellular location requires an intact N-terminus important for both MPK4 and WRKY33 interactions PMID: 21203436
    10. Mmutations of WRKY33 gene encoding a WRKY transcription factor cause enhanced susceptibility to Botrytis cinerea and Alternaria brassicicola concomitant with reduced expression of the jasmonate-regulated plant defensin PDF1.2 gene. PMID: 17059405
    11. Study shows that a set of three WRKY-specific cis-acting DNA elements (W boxes) within the AtWRKY33 promoter is required for efficient pathogen- or PAMP-triggered gene activation. PMID: 17427812

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    WRKY group I family
  • 組織特異性：
    Highly expressed in roots, leaves and flowers, and at lower levels in stems, siliques and seeds.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT2G38470

    STRING: 3702.AT2G38470.1

    UniGene: At.13123



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