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  4. Recombinant Arabidopsis thaliana Potassium transporter 5 (POT5), partial

Recombinant Arabidopsis thaliana Potassium transporter 5 (POT5), partial

  • 中文名稱：
    擬南芥POT5重組蛋白
  • 貨號：
    CSB-YP862994DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥POT5重組蛋白
  • 貨號：
    CSB-EP862994DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥POT5重組蛋白
  • 貨號：
    CSB-EP862994DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥POT5重組蛋白
  • 貨號：
    CSB-BP862994DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥POT5重組蛋白
  • 貨號：
    CSB-MP862994DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    POT5
  • Uniprot No.：
  • 別名：
    POT5; HAK5; At4g13420; T9E8.160; Potassium transporter 5; AtHAK1; AtHAK5; AtPOT5
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    High-affinity potassium transporter. Can also transport rubidium and cesium (Ref.1). Is essential with AKT1 for high-affinity potassium uptake in roots during seedling establishment and postgermination growth under low potassium conditions. Mediates potassium uptake by plant roots in response to low potassium conditions, by a calcium-, CBL-, and CIPK-dependent pathway. Positively regulated by the calcium sensors calcineurin B-like genes CBL1, CBL8, CBL9 and CBL10, and by phosphorylation by CIPK23.
  • 基因功能參考文獻(xiàn)：
    1. results demonstrate that ARF2 plays important roles in the response to external K(+) supply in Arabidopsis and regulates HAK5 transcription accordingly. PMID: 27895227
    2. ILK1 interacted with and promoted the accumulation of HAK5, a putative (H(+))/K(+) symporter that mediates a high-affinity uptake during K(+) deficiency.[ILK1] PMID: 27208244
    3. Data show that Arabidopsis Mutants lacking CBL-interacting protein kinase23 (CIPK23) are deficient in high-affinity K(+) transporter5 (HAK5)-mediated high-affinity potassium (K(+)) uptake. PMID: 26474642
    4. On the one hand the existence of a posttranscriptional regulation of HAK5 elicited by the low K(+) signal and on the other that HAK5 may be involved in yet-unknown functions related to NO(3)(-) and P deficiencies. PMID: 24716623
    5. Lack of ETO1 function leads to significant enhancement of tissue K status by an RBOHF-independent mechanism associated with elevated high-affinity K(+) TRANSPORTER5 transcript levels. PMID: 24064768
    6. HAK5 and four transcriptin factor genes DDF2, JLO, bHLH121 and TFII_A were highly up-regulated in response to both -K and salt stress. PMID: 23825216
    7. Although having a limited capacity, AtHAK5 plays a major role for K(+) acquisition from low K(+) concentrations in the presence of salinity. PMID: 20028724
    8. Results suggested that RCI3 is involved in the production of ROS under potassium deprivation and that RCI3-mediated ROS production affects the regulation of AtHAK5 expression. PMID: 20139158
    9. AtHAK5 is the only system mediating K+ uptake at concentrations below 0.01 mM. In the range between 0.01 and 0.05 mM K+ AtHAK5 and AtAKT1 are the only contributors to K+ acquisition. PMID: 20088908
    10. Upregulation of the HAK5 protein in response to potassium deprivation in A. thaliana. PMID: 15734909
    11. AtHAK5 is a root plasma membrane uptake mechanism for K(+) and Cs(+) under conditions of low K(+) availability. PMID: 18281719
    12. AtHAK5 mediates K+ absorption at low concentration better than AtAKT1. AtHAK5 induction is repressed by NH4+. PMID: 19000196

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    HAK/KUP transporter (TC 2.A.72.3) family
  • 組織特異性：
    Predominantly expressed in the roots.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT4G13420

    STRING: 3702.AT4G13420.1

    UniGene: At.3067



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