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  4. Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 2 (PLC2), partial

Recombinant Arabidopsis thaliana Phosphoinositide phospholipase C 2 (PLC2), partial

  • 中文名稱：
    擬南芥PLC2重組蛋白
  • 貨號：
    CSB-YP657184DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PLC2重組蛋白
  • 貨號：
    CSB-EP657184DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PLC2重組蛋白
  • 貨號：
    CSB-EP657184DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PLC2重組蛋白
  • 貨號：
    CSB-BP657184DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PLC2重組蛋白
  • 貨號：
    CSB-MP657184DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PLC2
  • Uniprot No.：
  • 別名：
    PLC2; At3g08510; T8G24.4; Phosphoinositide phospholipase C 2; EC 3.1.4.11; Phosphoinositide phospholipase PLC2; AtPLC2; PI-PLC2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. At physiological calcium concentration, the preferred substrate is phosphatidylinositol 4,5-bisphosphate versus phosphatidylinositol.
  • 基因功能參考文獻：
    1. The generation of reactive oxygen species (ROS) upon flg22 treatment is compromised in the PLC2-defficient plants, suggesting an effect of PLC2 in a branch of MAMP-triggered immunity and nonhost resistance that involves early ROS-regulated processes. Consistently, PLC2 associates with the NADPH oxidase RBOHD, suggesting its potential regulation by PLC2. PMID: 28827453
    2. essential for female gametogenesis and embryo development PMID: 27999988
    3. PLC2 is involved in auxin biosynthesis and signaling, thus modulating development of both male and female gametophytes in Arabidopsis. PMID: 26340337
    4. seedlings of plc2-1 mutant showed growth defect that was complemented by heterologous expression of AtPLC2, suggesting that phosphoinositide-specific phospholipase C activity borne by AtPLC2 is required for seedling growth PMID: 26401841
    5. AtPI4KIIIbeta1 and AtPI4KIIIbeta2 can both act upstream of the PI-PLC. There is evidence for the occurrence of a de novo synthesis of PtdIns4P by PI4K that coincides with PI-PLC activation. [PI-PLC] PMID: 22318862

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  • 亞細胞定位：
    Cell membrane; Peripheral membrane protein.
  • 組織特異性：
    Expressed in roots, shoots, leaves and flowers.
  • 數據庫鏈接：

    KEGG: ath:AT3G08510

    STRING: 3702.AT3G08510.1

    UniGene: At.20554



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