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  4. Recombinant Arabidopsis thaliana Nudix hydrolase 7 (NUDT7)

Recombinant Arabidopsis thaliana Nudix hydrolase 7 (NUDT7)

  • 中文名稱：
    擬南芥NUDT7重組蛋白
  • 貨號：
    CSB-YP890440DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NUDT7重組蛋白
  • 貨號：
    CSB-EP890440DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NUDT7重組蛋白
  • 貨號：
    CSB-EP890440DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NUDT7重組蛋白
  • 貨號：
    CSB-BP890440DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NUDT7重組蛋白
  • 貨號：
    CSB-MP890440DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NUDT7
  • Uniprot No.：
  • 別名：
    NUDT7; GFG1; NUDX7; At4g12720; T20K18.70; Nudix hydrolase 7; AtNUDT7; EC 3.6.1.-; ADP-ribose pyrophosphatase; EC 3.6.1.13; NADH pyrophosphatase; EC 3.6.1.22; Protein GROWTH FACTOR GENE 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-282
  • 氨基酸序列
    MGTRAQQIPL LEGETDNYDG VTVTMVEPMD SEVFTESLRA SLSHWREEGK KGIWIKLPLG LANLVEAAVS EGFRYHHAEP EYLMLVSWIS ETPDTIPANA SHVVGAGALV INKNTKEVLV VQERSGFFKD KNVWKLPTGV INEGEDIWTG VAREVEEETG IIADFVEVLA FRQSHKAILK KKTDMFFLCV LSPRSYDITE QKSEILQAKW MPIQEYVDQP WNKKNEMFKF MANICQKKCE EEYLGFAIVP TTTSSGKESF IYCNADHAKR LKVSRDQASA SL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can use both NADH and ADP-ribose as substrates, but not 8-oxo-dGTP, cyclic ADP-ribose, GDP-manose, UDP-glucose, ATP, or GTP. Exerts negative control of EDS1 signaling.
  • 基因功能參考文獻：
    1. The active and inactive AtNUDX6 proteins induced the expression of thioredoxin-h5 while the active and inactive AtNUDX7 proteins suppressed the accumulation of SA and subsequent gene expression, indicating that AtNUDX6 and 7 proteins themselves play distinct roles in stress responses. [AtNUDX7] PMID: 27095738
    2. provide evidence that AtERF1 is important for regulating AtNudt7 during oxidative signaling. PMID: 25451743
    3. AtNUDT7 is important for NAD:NADH homeostasis in seeds. PMID: 24504697
    4. NUDT7 forms complexes in vitro and in vivo with regulatory proteins involved in signal transduction. PMID: 22068106
    5. AtNUDT7 protein levels are induced in response to biotic and abiotic stresses. AtNUDT7 plays an important role in maintaining redox homeostasis, particularly for maintaining NADH:NAD+ balance for normal growth and development. PMID: 20704736
    6. Under oxidative stress, AtNUDX7 serves to maintain NAD(+) levels by supplying ATP via nucleotide recycling from free ADP-Rib molecules and thus regulates the defense mechanisms against oxidative DNA damage via modulation of the PAR reaction. [AtNUDX7] PMID: 19656905
    7. GFG1 is a key gene linking cellular metabolism and oxidative signaling. PMID: 16328543
    8. FMO1 and NUDT7 are important for signaling through an EDS1-dependent but SA-independent branch of plant defense and NUDT7 exerts negative control of EDS1 signaling. [NUDT7] PMID: 16531493
    9. functions to modulate the defense response to prevent excessive stimulation, ADP-ribose and NADH are preferred substrates PMID: 17660350
    10. structure-activity studies: mutation of specific residues in AtNUDT7 alters function; mutation of residues F73 & V168 disallows dimer formation & enzymatic activity; mutation of residues V69, F73, & V168 disrupts binding to regulatory 14-3-3 protein PMID: 19448856

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  • 亞細胞定位：
    Nucleus. Cytoplasm. Cell membrane. Note=Localized at the plasma membrane when in complex with GG2, but present in the cytoplasm when associated with GG1. Detected in the cytoplasm and nucleus when interacting with RACK1A.
  • 蛋白家族：
    Nudix hydrolase family
  • 組織特異性：
    Expressed in stems, leaves, roots, flowers and siliques.
  • 數據庫鏈接：

    KEGG: ath:AT4G12720

    UniGene: At.23449



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