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  4. Recombinant Arabidopsis thaliana Nitric oxide synthase 1 (NOS1), partial

Recombinant Arabidopsis thaliana Nitric oxide synthase 1 (NOS1), partial

  • 中文名稱：
    擬南芥NOA1重組蛋白
  • 貨號：
    CSB-YP721062DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NOA1重組蛋白
  • 貨號：
    CSB-EP721062DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NOA1重組蛋白
  • 貨號：
    CSB-EP721062DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NOA1重組蛋白
  • 貨號：
    CSB-BP721062DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NOA1重組蛋白
  • 貨號：
    CSB-MP721062DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NOA1
  • Uniprot No.：
  • 別名：
    NOA1; NOS1; RIF1; At3g47450; T21L8.200; NO-associated protein 1; chloroplastic/mitochondrial; AtNOA1; Dubious mitochondrial nitric oxide synthase 1; AtNOS1; EC 1.14.13.39; GTPase NOA1; Protein RESISTANT TO INHIBITION BY FOSMIDOMYCIN 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Exhibits cGTPase activity; binds and hydrolyzes specifically GTP. May participate in ribosome assembly and stability and thus regulates protein synthesis in chloroplasts. The GTPase activity requires MgCl(2)and the presence of either KCl or (NH(4))(2)SO(4). Involved in the post-transcriptional regulation of the methylerythritol phosphate (MEP) pathway. Involved in chlorophyll-a fluorescence regulation.; May mediate the production or accumulation of nitric oxide (NO) which is a messenger molecule involved in hormonal signaling and defense responses in plant. Acts as an antisenescence agent. Plays a crucial role in both extracellular calmodulin (ExtCaM)-triggered and salicylic acid (SA)-mediated H(2)O(2)-dependent stomatal closure.
  • 基因功能參考文獻：
    1. in response to carbon dioxide elevation, either the NR activity induction in low-nitrate plants or the NR activity inhibition in high-nitrate plants is regulated by NOS-generated nitric oxide. PMID: 26608644
    2. The protein S-acyltransferases PAT13 and PAT14 are involved in leaf senescence control- possibly via NOA1 S-acylation-, providing a new sight into the regulation mechanism of S-acylation in leaf senescence. PMID: 26160582
    3. Salicylic acid signaling through NPR1 and AtNOA1 is involved in the control of root waving. PMID: 25690466
    4. NOA1 has a primary role in chloroplast function and its effects on the accumulation of nitric oxide are likely to be indirect. PMID: 21689173
    5. NIA1/2/NOA1-dependent NO production mediates Arabidopsis salt tolerance. PMID: 23744476
    6. Oleic levels regulate nitric oxide(NO) synthesis, and, thereby, NO-mediated signaling, by regulating NOA1 levels. PMID: 22492810
    7. AtNOA1 regulates salicylic acid-induced NO production and stomatal closure. PMID: 20657186
    8. Atnoa1 mutant has higher salicylic acid content than wild-type Arabidopsis. PMID: 21392840
    9. A highly conserved nuclear-encoded cGTPase of likely prokaryotic origin is essential for proper chloroplast ribosome assembly and/or translation in plants. PMID: 20456051
    10. NOA1/nitrate reductase triple mutants are severely impaired in nitric oxide production. PMID: 20007448
    11. Atnos1 mutant plants show increased hypersensitivity to salt stress and methyl viologen (MV) treatment. PMID: 16690168
    12. The results of studies concerning the apparent function of ATNOS1 are reported and discussed. PMID: 17030145
    13. AtNOS1 is involved in salt tolerance in Arabidopsis. PMID: 17351048
    14. At3g47450 was found to be a plastid-targeted homolog of the Bacillus subtilis YqeH protein, a GTPase required for proper ribosome assembly, and in mutant seedlings, decreased levels of plastome-encoded proteins were observed. PMID: 18469163
    15. AtNOS/AtNOA1 is a functional Arabidopsis thaliana cGTPase and not a nitric-oxide synthase PMID: 18801746
    16. YqeH and AtNOA1 probably act as G-proteins that regulate nucleic acid recognition and not as nitric-oxide synthases PMID: 18801747
    17. NITRIC OXIDE ASSOCIATED1 (AtNOA1)-dependent nitric oxide (NO) accumulation plays a crucial role in Extracellular calmodulin-induced stomatal closure. PMID: 19321706

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  • 亞細胞定位：
    Mitochondrion. Plastid, chloroplast.
  • 蛋白家族：
    TRAFAC class YlqF/YawG GTPase family, NOA1 subfamily
  • 組織特異性：
    Expressed in aleurone layer and the embryo.
  • 數據庫鏈接：

    KEGG: ath:AT3G47450

    STRING: 3702.AT3G47450.1

    UniGene: At.43334



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