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  4. Recombinant Arabidopsis thaliana Mitogen-activated protein kinase kinase kinase 1 (MEKK1), partial

Recombinant Arabidopsis thaliana Mitogen-activated protein kinase kinase kinase 1 (MEKK1), partial

  • 中文名稱：
    擬南芥MEKK1重組蛋白
  • 貨號：
    CSB-YP013417DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MEKK1重組蛋白
  • 貨號：
    CSB-EP013417DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MEKK1重組蛋白
  • 貨號：
    CSB-EP013417DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MEKK1重組蛋白
  • 貨號：
    CSB-BP013417DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MEKK1重組蛋白
  • 貨號：
    CSB-MP013417DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MEKK1
  • Uniprot No.：
  • 別名：
    MEKK1; At4g08500; T15F16.5Mitogen-activated protein kinase kinase kinase 1; ARAKIN; AtMEKK1; MAP kinase kinase kinase 1; EC 2.7.11.25
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MKK1/MKK2 and MPK4/MPK6). Activates by phosphorylation the downstream MKK2, MKK4 and MKK5 in a calcium-dependent manner.
  • 基因功能參考文獻：
    1. Treatment of Arabidopsis with a membrane rigidifier, DMSO, causes MPK4 activation concomitantly with MEKK1 and MKK2 phosphorylation. PMID: 24801600
    2. Ca(2+) signaling occurred upstream of the MEKK1-MKK2 pathway. MEKK1 was phosphorylated by calcium/calmodulin-regulated receptor-like kinase (CRLK1), which suggested that CRLK1 is one of candidates located upstream of MEKK1. PMID: 23857079
    3. MEKK1 plays a key role in transducing the l-Glu signal that elicits large-scale changes in root architecture, and provide genetic evidence for the existence in plants of an external glutamate (l-Glu) signalling pathway analogous to that found in animals. PMID: 23574009
    4. Data suggest that the MEKK1-MKK1/MKK2-MPK4 kinase cascade negatively regulates MEKK2 and activation of MEKK2 triggers SUMM2-mediated immune responses. PMID: 22643122
    5. CRLK1 interacts with MEKK1 in vitro and in planta during cold treatment. PMID: 20724845
    6. An analysis of the interation of MEKK1 and MEK1 in response to wounding stress in A. thaliana seedlings is presented. PMID: 16211390
    7. MEKK1 is essential for activation of MPK4 and negatively regulates temperature-sensitive and tissue-specific cell death and H(2)O(2) accumulation that are dependent on both RAR1 (resistance protein function) and SID2 (isochorismate synthase) PMID: 17023433
    8. This study demonstrated that analysis of plants carrying T-DNA knockout alleles indicated that MEKK1 is required for flg22-induced activation of MPK4. PMID: 17142480
    9. Treatment with elevated levels of sodium chloride improves the growth of mekk1 transgenic plants;the mekk1;mpk4 double-mutant combination causes seedling lethality. PMID: 17572407
    10. MEKK1 is a bifunctional protein: it binds to the promoter of the WRKY53 gene regulating the switch from a leaf age dependent to a plant age dependent expression PMID: 17587183
    11. Activation of MPK4 by flg22 is impaired in the mkk1 mkk2 double mutants, suggesting that MKK1 and MKK2 function together with MPK4 and MEKK1 in a MAP kinase cascade to negatively regulate innate immune responses in plants. PMID: 18982020

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  • 亞細胞定位：
    Cell membrane. Endosome.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily
  • 數據庫鏈接：

    KEGG: ath:AT4G08500

    STRING: 3702.AT4G08500.1

    UniGene: At.21066



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